Best practices for preparing a single cell suspension from solid tissues for flow cytometry
A Reichard, K Asosingh - Cytometry Part A, 2019 - Wiley Online Library
Preparing a single cell suspension is a critical step in any solid tissue flow cytometry
experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three …
experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three …
Single cell analysis of human tissues and solid tumors with mass cytometry
Background Mass cytometry measures 36 or more markers per cell and is an appealing
platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While …
platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While …
Flow cytometry and cell sorting
SF Ibrahim, G van den Engh - Cell separation: fundamentals, analytical …, 2007 - Springer
Flow cytometry and cell sorting are well-established technologies in clinical diagnostics and
biomedical research. Heterogeneous mixtures of cells are placed in suspension and passed …
biomedical research. Heterogeneous mixtures of cells are placed in suspension and passed …
Collection, storage, and preparation of human blood cells
PK Dagur, JP McCoy Jr - Current protocols in cytometry, 2015 - Wiley Online Library
Human peripheral blood is often studied by flow cytometry in both the research and clinical
laboratories. The methods for collection, storage, and preparation of peripheral blood will …
laboratories. The methods for collection, storage, and preparation of peripheral blood will …
[图书][B] Flow cytometry
MG Macey, MG Macey - 2007 - Springer
Improvements in instrument design and computing power and the increased availability of
fluorescent agents have led to an increased usage of flow cytometry in both the research …
fluorescent agents have led to an increased usage of flow cytometry in both the research …
Flow cytometry: retrospective, fundamentals and recent instrumentation
Flow cytometry is a complete technology given to biologists to study cellular populations with
high precision. This technology elegantly combines sample dimension, data acquisition …
high precision. This technology elegantly combines sample dimension, data acquisition …
Flow cytometry: present and future
KA Muirhead, PK Horan, G Poste - Bio/technology, 1985 - nature.com
Flow cytometry combines many of the advantages of microscopy and biochemical analysis
in a single high precision technique for rapid phenotypic analysis and sorting of individual …
in a single high precision technique for rapid phenotypic analysis and sorting of individual …
Flow cytometry: basic principles and applications
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a
single cell such as size and granularity simultaneously as the cell flows in suspension …
single cell such as size and granularity simultaneously as the cell flows in suspension …
The Parsortix™ cell separation system—A versatile liquid biopsy platform
MC Miller, PS Robinson, C Wagner… - Cytometry Part …, 2018 - Wiley Online Library
Cancer cells from solid tumors can enter the circulatory system and survive to subsequently
form distant metastases. The CellSearch® system (Menarini‐Silicon Biosystems …
form distant metastases. The CellSearch® system (Menarini‐Silicon Biosystems …
Automated flow cytometry for acquisition of time‐dependent population data
NR Abu‐Absi, A Zamamiri, J Kacmar… - Cytometry Part A …, 2003 - Wiley Online Library
Background The implementation of flow cytometry in many experimental settings can be
limited by the extensive amount of sample handling and preparation required for analysis …
limited by the extensive amount of sample handling and preparation required for analysis …