Best practices for preparing a single cell suspension from solid tissues for flow cytometry

A Reichard, K Asosingh - Cytometry Part A, 2019 - Wiley Online Library
Preparing a single cell suspension is a critical step in any solid tissue flow cytometry
experiment. Tissue dissection, enzymatic digestion, and mechanical dissociation are three …

Single cell analysis of human tissues and solid tumors with mass cytometry

N Leelatian, DB Doxie, AR Greenplate… - Cytometry Part B …, 2017 - Wiley Online Library
Background Mass cytometry measures 36 or more markers per cell and is an appealing
platform for comprehensive phenotyping of cells in human tissue and tumor biopsies. While …

Flow cytometry and cell sorting

SF Ibrahim, G van den Engh - Cell separation: fundamentals, analytical …, 2007 - Springer
Flow cytometry and cell sorting are well-established technologies in clinical diagnostics and
biomedical research. Heterogeneous mixtures of cells are placed in suspension and passed …

Collection, storage, and preparation of human blood cells

PK Dagur, JP McCoy Jr - Current protocols in cytometry, 2015 - Wiley Online Library
Human peripheral blood is often studied by flow cytometry in both the research and clinical
laboratories. The methods for collection, storage, and preparation of peripheral blood will …

[图书][B] Flow cytometry

MG Macey, MG Macey - 2007 - Springer
Improvements in instrument design and computing power and the increased availability of
fluorescent agents have led to an increased usage of flow cytometry in both the research …

Flow cytometry: retrospective, fundamentals and recent instrumentation

J Picot, CL Guerin, C Le Van Kim, CM Boulanger - Cytotechnology, 2012 - Springer
Flow cytometry is a complete technology given to biologists to study cellular populations with
high precision. This technology elegantly combines sample dimension, data acquisition …

Flow cytometry: present and future

KA Muirhead, PK Horan, G Poste - Bio/technology, 1985 - nature.com
Flow cytometry combines many of the advantages of microscopy and biochemical analysis
in a single high precision technique for rapid phenotypic analysis and sorting of individual …

Flow cytometry: basic principles and applications

A Adan, G Alizada, Y Kiraz, Y Baran… - Critical reviews in …, 2017 - Taylor & Francis
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a
single cell such as size and granularity simultaneously as the cell flows in suspension …

The Parsortix™ cell separation system—A versatile liquid biopsy platform

MC Miller, PS Robinson, C Wagner… - Cytometry Part …, 2018 - Wiley Online Library
Cancer cells from solid tumors can enter the circulatory system and survive to subsequently
form distant metastases. The CellSearch® system (Menarini‐Silicon Biosystems …

Automated flow cytometry for acquisition of time‐dependent population data

NR Abu‐Absi, A Zamamiri, J Kacmar… - Cytometry Part A …, 2003 - Wiley Online Library
Background The implementation of flow cytometry in many experimental settings can be
limited by the extensive amount of sample handling and preparation required for analysis …