Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L

Y Wang, Y Zhang, Q Liu, H Tong, T Zhang, C Gu… - Scientific Reports, 2021 - nature.com
Iris germanica L. is a perennial herbaceous plant that has been widely cultivated worldwide
and is popular for its elegant and vibrantly colorful flowers. Selection of appropriate …

[PDF][PDF] Reference gene selection for qRT-PCR normalization in Iris germanica L.

Y Wang, Y Zhang, Q Liu, L Liu, S Huang, H Yuan - 2020 - cdn.techscience.cn
Quantitative real-time PCR (qPCR) is an effective and widely used method to analyze
expression patterns of target genes. Selection of stable reference genes is a prerequisite for …

Reference Gene Selection for Quantitative Real‐Time RT‐PCR Normalization in Iris. lactea var. chinensis Roots under Cadmium, Lead, and Salt Stress Conditions

CS Gu, L Liu, C Xu, Y Zhao, X Zhu… - The Scientific World …, 2014 - Wiley Online Library
Quantitative real time PCR (RT‐qPCR) has emerged as an accurate and sensitive method to
measure the gene expression. However, obtaining reliable result depends on the selection …

Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz

S Yi, Y Qian, L Han, Z Sun, C Fan, J Liu, G Ju - Scientia Horticulturae, 2012 - Elsevier
Selection of reliable reference genes is a prerequisite for accurate normalization of gene
expression in quantitative real-time polymerase chain reaction (qRT-PCR). The present …

Investigation of endogenous phytohormones and critical flowering genes during flower development in Iris germanica

X Zhao, Y Wu, J Qian, G Bian, X Zhang, B Su, Y Tian… - Scientia …, 2024 - Elsevier
With showy display and multi-color, Iris germanica (Bearded iris) is one of the most popular
ornamental perennials, of which molecular mechanisms in regulation of flowering are poorly …

Reference Gene Selection for RT-qPCR Analysis of Flower Development in Chrysanthemum morifolium and Chrysanthemum lavandulifolium

S Qi, L Yang, X Wen, Y Hong, X Song… - Frontiers in plant …, 2016 - frontiersin.org
Quantitative real-time PCR (qPCR) is a popular and powerful tool used to understand the
molecular mechanisms of flower development. However, the accuracy of this approach …

Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR

XY Li, JY Cheng, J Zhang, JA Teixeira da Silva… - PloS one, 2015 - journals.plos.org
Lilium is an important commercial market flower bulb. qRT-PCR is an extremely important
technique to track gene expression levels. The requirement of suitable reference genes for …

Selection and Validation of Candidate Reference Genes for Gene Expression Analysis by RT-qPCR in Rubus

Y Wu, C Zhang, H Yang, L Lyu, W Li, W Wu - International Journal of …, 2021 - mdpi.com
Due to the lack of effective and stable reference genes, studies on functional genes in
Rubus, a genus of economically important small berry crops, have been greatly limited. To …

Validation of Reference Genes for Quantitative Real-Time PCR during Bicolor Tepal Development in Asiatic Hybrid Lilies (Lilium spp.)

L Xu, H Xu, Y Cao, P Yang, Y Feng, Y Tang… - Frontiers in plant …, 2017 - frontiersin.org
Quantitative real-time PCR (qRT-PCR) is a reliable and high-throughput technique for gene
expression studies, but its accuracy depends on the expression stability of reference genes …

Identification of Suitable Reference Genes for RT-qPCR Assays in Liriodendron chinense (Hemsl.) Sarg

Z Tu, Z Hao, W Zhong, H Li - Forests, 2019 - mdpi.com
The precision and reliability of reverse transcription quantitative polymerase chain reaction
(RT-qPCR) depend mainly on suitable reference genes; however, reference genes have not …