Prime editing is a highly versatile CRISPR-based genome editing technology that works
without DNA double-strand break formation. Despite rapid technological advances, in vivo …

CRISPR–Cas-based genome editing holds great promise for targeting genetic disorders,
including inborn errors of hepatocyte metabolism. Precise correction of disease-causing …

Large scale genomic aberrations including duplication, deletion, translocation, and other
structural changes are the cause of a subtype of hereditary genetic disorders and contribute …

An article published in Nature (Anzalone et al., 2019) reports the development of a genome
editing experimental approach that mediates all possible base-to-base conversions,“indels,” …

Delivery and optimization of prime editors (PEs) have been hampered by their large size
and complexity. Although split versions of genome-editing tools can reduce construct size …

Gene therapy holds tremendous potential in the treatment of inherited diseases. Unlike
traditional medicines, which only treat the symptoms, gene therapy has the potential to cure …

Prime editors (PEs) mediate genome modification without utilizing double-stranded DNA
breaks or exogenous donor DNA as a template. PEs facilitate nucleotide substitutions or …

Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR–
Cas)-mediated genome editing has revolutionized biomedical research and will likely …

Prime editing is a recently reported genome editing tool using a nickase-cas9 fused to a
reverse transcriptase that directly synthesizes the desired edit at the target site. Here, we …

Genetically engineered mouse models only capture a small fraction of the genetic lesions
that drive human cancer. Current CRISPR–Cas9 models can expand this fraction but are …