A method whereby DNA sequences of varying size may be accurately inserted into pre-
determined locations within the Escherichia coli genome was developed. Using a binary …

Fig. 1: Recombineering. A, Double homologous recombination between DNA molecules
results in an exchange of the genetic information which is flanked by similar or identical …

We describe an optimized system for the easy, effective, and precise modification of the
Escherichia coli genome. Genome changes are introduced first through the integration of a …

The present invention relates to a recombinant E. coli exhib iting a complex phenotype,
comprising three or more heter ologous DNA fragments integrated into a chromosome of the …

We have developed an improved and rapid genomic engineering procedure for the
construction of custom-designed microorganisms. This method, which can be performed in 2 …

Numerous genome information was obtained using the result of genome sequences of
many microorganisms, functional analyses of the genomes, comparative genomics, and …

Transformation is defined as the transfer of genetic information into a recipient bacterium
using naked DNA, without any requirement for contact with a donor bacterium. The ability to …

A systematic approach based on bacteriophage Lambda (Lambda Red) and flippase-
flippase recognition targets (FLP-FRT) recombinations was proposed for genomic …

Fluent genetic manipulation of prokaryote genomes is still limited to only a few commonly
used hosts. Ideally the advanced technologies available for cloning into recombinant …

Various methods have been developed for gene disruption in bacteria; however, extra in
vitro manipulation steps or the residual presence of a scar in the host chromosome limits the …