The bacterium Escherichia coli is a major host for recombinant protein production of non-
glycosylated products. Depending on the expression strategy, the recombinant protein can …

Background The bacterium E. coli is a major host for recombinant protein production of non-
glycosylated products. Depending on the expression strategy, the recombinant protein can …

Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive
aggregates of misfolded protein, and thus should be avoided during recombinant protein …

The overexpression of recombinant proteins in Escherichia coli leads in most cases to their
accumulation in the form of insoluble aggregates referred to as inclusion bodies (IBs). To …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

Bacterial inclusion bodies (IBs) are refractile aggregates of protease-resistant misfolded
protein that often occur in recombinant bacteria upon gratuitous overexpression of cloned …

Human G‐CSF (granulocyte colony‐stimulating factor) is a well‐known biopharmaceutical
drug being mostly produced by overexpression in Escherichia coli, where it appears in the …

Inclusion bodies (IBs) are insoluble aggregates of misfolded protein in Escherichia coli.
Against the outdated belief that the production of IBs should be avoided during recombinant …