The underpinnings of cancer metastasis remain poorly understood, in part due to a lack of
tools for probing their emergence at high resolution. Here we present macsGESTALT, an …

INTRODUCTION Cancer progression is fundamentally an evolutionary process, involving
multiple distinct steps from oncogenic transformation to metastatic dissemination to …

While CRISPR screens are helping uncover genes regulating many cell-intrinsic processes,
existing approaches are suboptimal for identifying extracellular gene functions, particularly …

Multimodal single-cell assays provide high-resolution snapshots of complex cell
populations, but are mostly limited to transcriptome plus an additional modality. Here, we …

Systematic investigation of the genetic interactions that influence metastatic potential has
been challenging. Here we developed massively parallel CRISPR–Cpf1/Cas12a crRNA …

Cancer genomics consortia have charted the landscapes of numerous human cancers.
Whereas some mutations were found in classical oncogenes and tumor suppressors, others …

Intratumor genetic heterogeneity underlies the ability of tumors to evolve and adapt to
different environmental conditions. Using CRISPR/Cas9 technology and specific DNA …

Chromatin accessibility profiling can identify putative regulatory regions genome wide;
however, pooled single-cell methods for assessing the effects of regulatory perturbations on …

Genetic screens are powerful tools for identifying genes responsible for diverse phenotypes.
Here we describe a genome-wide CRISPR/Cas9-mediated loss-of-function screen in tumor …

Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited
by the long duration of allele/model generation. Here we show transfection-based …