Thermostable proteins find their use in numerous biomedical and biotechnological
applications. However, the computational design of stable proteins often results in single …

There is a continuous interest in increasing proteins stability to enhance their usability in
numerous biomedical and biotechnological applications. A number of in silico tools for the …

Many proteins have low thermodynamic stability, which can lead to low expression yields
and limit functionality in research, industrial and clinical settings. This article introduces two …

Designing proteins with enhanced thermo‐stability has been a main focus of protein
engineering because of its theoretical and practical significance. Despite extensive studies …

Natural proteins are often only slightly more stable in the native state than the denatured
state, and an increase in environmental temperature can easily shift the balance toward …

The rational design of enzymes is a challenging research field, which plays an important
role in the optimization of a wide series of biotechnological processes. Computational …

There is great interest in increasing proteins' stability to enhance their utility as biocatalysts,
therapeutics, diagnostics and nanomaterials. Directed evolution is a powerful, but …

Highlights•Targeting critical sites for stability improvement.•Consensus/ancestral sequence
based protein stabilization.•Computational design of thermostability.•Loop grafting and …

Many natural and designed proteins are only marginally stable limiting their usefulness in
research and applications. Recently, we described an automated structure and sequence …

The stability of wild-type proteins is often a hurdle to their practical use in research, industry,
and medicine. The route to engineering stability of a protein of interest lies largely with the …