The pathway of anaerobic toluene degradation is initiated by a remarkable radical-type
enantiospecific addition of the chemically inert methyl group to the double bond of a …

Anaerobic toluene degradation is initiated by adding toluene to fumarate via the glycyl
radical enzyme benzylsuccinate synthase (BSS). We developed a recombinant production …

Various bacteria perform anaerobic degradation of small hydrocarbons as a source of
energy and cellular carbon. To activate non-reactive hydrocarbons such as toluene …

Density functional theory calculations using the hybrid functional B3LYP have been
performed to study the catalytic mechanism of benzylsuccinate synthase. This enzyme …

Benzylsuccinate synthase is a member of the glycyl radical family of enzymes. It catalyzes
the addition of toluene to fumarate to form benzylsuccinate as the first step in the anaerobic …

Toluene is anoxically degraded to CO2 by the denitrifying bacterium Thauera aromatica.
The initial reaction in this pathway is the addition of fumarate to the methyl group of toluene …

Anaerobic degradation of the environmental pollutant toluene is initiated by the glycyl
radical enzyme benzylsuccinate synthase (BSS), which catalyzes the radical addition of …

The first step in the anaerobic metabolism of toluene is a highly unusual reaction: the
addition of toluene across the double bond of fumarate to produce (R)-benzylsuccinate …

Molecular modeling techniques and density functional theory calculations were performed to
study the mechanism of enzymatic radical C–C coupling catalyzed by benzylsuccinate …

The fumarate addition reaction, catalyzed by the enzyme benzylsuccinate synthase (BSS), is
considered to be one of the most intriguing and energetically challenging reactions in …