Development of a validation protocol of enzyme immunoassay kits used for the analysis of steroid hormones in fish plasma

SS Metcalfe, FJ Kroon, DJ Beale, G Miller - Journal of Experimental Marine …, 2018 - Elsevier
The analysis of steroid hormones 17β-estradiol, testosterone and 11-ketotestosterone in fish
plasma provide insight into fish reproductive behavior and development. Typically, steroid …

Plasma cortisol and 11‐ketotestosterone enzyme immunoassay (EIA) kit validation for three fish species: the orange clownfish Amphiprion percula, the orangefin …

SC Mills, J Mourier, R Galzin - Journal of Fish Biology, 2010 - Wiley Online Library
Commercially available enzyme immunoassay (EIA) kits were validated for measuring
steroid hormone concentrations in blood plasma from three fish species: the orange …

Quantification of steroid hormones in low volume plasma and tissue homogenates of fish using LC-MS/MS

MZ Nouri, KJ Kroll, M Webb, ND Denslow - General and comparative …, 2020 - Elsevier
Quantification of steroid hormones in fish is an important step for toxicology and
endocrinology studies. Among the hormone analysis techniques, liquid chromatography …

Steroid determination in fish plasma using capillary electrophoresis

L Bykova, SA Archer‐Hartmann… - Environmental …, 2010 - Wiley Online Library
A capillary separation method that incorporates pH‐mediated stacking is employed for the
simultaneous determination of circulating steroid hormones in plasma from Perca flavescens …

Measuring multiple hormones from a single water sample using enzyme immunoassays

CE Kidd, MR Kidd, HA Hofmann - General and comparative endocrinology, 2010 - Elsevier
Many aquatic species, such as teleosts, release into the water and detect multiple bioactive
substances to assist in schooling, migration, alarm reactions, and to stimulate behavioral …

Blood steroid levels in the goldfish: Measurement of six ovarian steroids in small volumes of serum by reverse-phase high-performance liquid chromatography and …

B Venkatesh, CH Tan, TJ Lam - General and comparative endocrinology, 1989 - Elsevier
A reliable and rapid technique for the measurement of estradiol-17β, testosterone,
progesterone, 17α-hydroxyprogesterone, 17α, 20β-dihydroxy-4-pregnen-3-one, and cortisol …

High-sensitivity determination of estrogens in fish plasma using chemical derivatization upstream UHPLC–MSMS

U Bussy, YW Chung-Davidson, TJ Buchinger, K Li… - Steroids, 2017 - Elsevier
This article describes the development and validation of a sensitive LC–MSMS method for
determination of estrogen in fish plasma. Dansyl chloride derivatization of the phenol …

[PDF][PDF] Why measure steroids in fish plasma when you can measure them in water

AP Scott, M Pinillos, T Ellis - … unity and diversity: the proceedings of …, 2001 - researchgate.net
The measurement of steroid concentrations in water rather than in blood plasma, has
potential in a number of situations: to study steroidal pheromones; to measure sequential …

Ultra-performance liquid chromatography tandem mass spectrometry for simultaneous determination of natural steroid hormones in sea lamprey (Petromyzon marinus …

H Wang, U Bussy, YW Chung-Davidson, W Li - Journal of Chromatography …, 2016 - Elsevier
This study aims to provide a rapid, sensitive and precise UPLC–MS/MS method for target
steroid quantitation in biological matrices. We developed and validated an UPLC–MS/MS …

Simultaneous quantitation of testosterone, estradiol, ethinyl estradiol, and 11‐ketotestosterone in fathead minnow fish plasma by liquid chromatography/positive …

F Zhang, MJ Bartels, DR Geter, MS Carr… - … Journal Devoted to …, 2009 - Wiley Online Library
In the present work, for the first time, a rapid and sensitive liquid chromatography/positive
atmospheric pressure photoionization tandem mass spectrometry (LC/APPI‐MS/MS) method …