Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro

JE Swain, CL Bormann, SG Clark, EM Walters… - …, 2002 - rep.bioscientifica.com
The effects of four reagents on the activation and subsequent fertilization of equine oocytes,
and the development of these after intracytoplasmic sperm injection, were investigated …

Effect of short periods of sperm–oocyte coincubation during in vitro fertilization on embryo development in pigs

MA Gil, M Ruiz, JM Vazquez, J Roca, BN Day… - Theriogenology, 2004 - Elsevier
The present study was conducted to determine if the efficiency of in vitro pig embryo
production could be improved by a reduction in the period of time that oocytes are exposed …

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

D Rath - Theriogenology, 1992 - Elsevier
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …

Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection

MJ Martin - Biology of Reproduction, 2000 - academic.oup.com
The objective of this study was to assess the development of porcine ova fertilized by
intracytoplasmic sperm injection (ICSI). Allyl trenbolone (Regumate) was used to …

Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa

LR Abeydeera, BN Day - Biology of reproduction, 1997 - academic.oup.com
The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …

Comparisons of oocyte maturation times and of three methods of sperm preparation for their effects on the production of goat embryos in vitro

GJ Rho, AC Hahnel, KJ Betteridge - Theriogenology, 2001 - Elsevier
Various times of in vitro maturation of oocytes, and three methods of separating
spermatozoa from frozen-thawed semen (Percoll density-gradient centrifugation, swim-up …

Procedural improvements for in vitro production of viable uterine stage caprine embryos

L Keskintepe, GC Luvoni, SJ Rzucidlo… - Small Ruminant …, 1996 - Elsevier
Efforts to improve proportions of caprine immature oocytes developing into viable uterine-
stage embryos in vitro involved study of 1924 oocytes in experiments designed to examine …

Viable piglets generated from porcine oocytes matured in vitro and fertilized by intracytoplasmic sperm head injection

M Nakai, N Kashiwazaski, A Takizawa… - Biology of …, 2003 - academic.oup.com
Intracytoplasmic sperm injection (ICSI) of a nonmotile cell into the ooplasm for assisted
fertilization is a highly specialized procedure for producing the next generation. The …

Effect of sperm exposure time on in vitro fertilization and embryo development of bovine oocytes matured in vitro

N Rehman, AR Collins, TK Suh, RW Wright Jr - Theriogenology, 1994 - Elsevier
This study was designed to investigate the effect of sperm exposure time on the fertilization
rate and subsequent developmental capacity of bovine oocytes matured in vitro. Cumulus …

Maturation, fertilization and complete development of porcine oocytes matured under different systems

P Coy, S Ruiz, R Romar, I Campos, J Gadea - Theriogenology, 1999 - Elsevier
This study was designed 1) to determine the effectiveness of 2 in vitro maturation systems
commonly employed to produce nuclear and cytoplasmicly mature pig oocytes, 2) to assess …