Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements

J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Strategies for optimizing heterologous protein expression in Escherichia coli

G Hannig, SC Makrides - Trends in biotechnology, 1998 - cell.com
The many advantages of Escherichia coli have ensured that it remains a valuable host for
the efficient, cost-effective and high-level production of heterologous proteins. Here, we …

Advanced genetic strategies for recombinant protein expression in Escherichia coli

HP Sørensen, KK Mortensen - Journal of biotechnology, 2005 - Elsevier
Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …

Strategies to Optimize Protein Expression in E. coli

DM Francis, R Page - Current protocols in protein science, 2010 - Wiley Online Library
Recombinant protein expression in Escherichia coli (E. coli) is simple, fast, inexpensive, and
robust, with the expressed protein comprising up to 50 percent of the total cellular protein …

Optimization of heterologous protein production in Escherichia coli

MJ Weickert, DH Doherty, EA Best, PO Olins - Current opinion in …, 1996 - Elsevier
Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous
proteins. Recent advances have been made in the expression of complex proteins as …

Recombinant protein expression in Escherichia coli (E. coli): what we need to know

SMG Hayat, N Farahani, B Golichenari… - Current …, 2018 - ingentaconnect.com
Background: Host, vector, and culture conditions (including cultivation media) are
considered among the three main elements contributing to a successful production of …

Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Genome engineering for improved recombinant protein expression in Escherichia coli

S Mahalik, AK Sharma, KJ Mukherjee - Microbial cell factories, 2014 - Springer
A metabolic engineering perspective which views recombinant protein expression as a
multistep pathway allows us to move beyond vector design and identify the downstream rate …

[PDF][PDF] An overview of the parameters for recombinant protein expression in Escherichia coli

BC Joseph, S Pichaimuthu, S Srimeenakshi… - J Cell Sci …, 2015 - researchgate.net
Escherichia coli, is one of the most widely preferred organism for the production of
recombinant protein. Most of the FDA approved therapeutic proteins are produced in E. coli …

Strategies for the Production of Recombinant Protein in Escherichia coli

GJ Gopal, A Kumar - The protein journal, 2013 - Springer
In the recent past years, a large number of proteins have been expressed in Escherichia coli
with high productivity due to rapid development of genetic engineering technologies. There …