Engineering circular RNA for enhanced protein production
Circular RNAs (circRNAs) are stable and prevalent RNAs in eukaryotic cells that arise from
back-splicing. Synthetic circRNAs and some endogenous circRNAs can encode proteins …
back-splicing. Synthetic circRNAs and some endogenous circRNAs can encode proteins …
Engineering circular RNA for potent and stable translation in eukaryotic cells
RA Wesselhoeft, PS Kowalski, DG Anderson - Nature communications, 2018 - nature.com
Messenger RNA (mRNA) has broad potential for application in biological systems. However,
one fundamental limitation to its use is its relatively short half-life in biological systems. Here …
one fundamental limitation to its use is its relatively short half-life in biological systems. Here …
Circular RNA: Biosynthesis in vitro
X Chen, Y Lu - Frontiers in bioengineering and biotechnology, 2021 - frontiersin.org
Circular RNA (circRNA) is a unique type of noncoding RNA molecule. Compared with
traditional linear RNA, circRNA is a covalently closed circle produced by a process called …
traditional linear RNA, circRNA is a covalently closed circle produced by a process called …
Engineering highly efficient backsplicing and translation of synthetic circRNAs
Circular RNAs (circRNAs) are highly stable RNA molecules that are attractive templates for
expression of therapeutic proteins and non-coding RNAs. In eukaryotes, circRNAs are …
expression of therapeutic proteins and non-coding RNAs. In eukaryotes, circRNAs are …
The design and synthesis of circular RNAs
P Obi, YG Chen - Methods, 2021 - Elsevier
Circular RNAs (circRNAs) are a novel class of RNAs distinguished by their single-stranded,
covalently-closed topology. Although initially perceived as rare byproducts of aberrant …
covalently-closed topology. Although initially perceived as rare byproducts of aberrant …
RNA circularization strategies in vivo and in vitro
S Petkovic, S Müller - Nucleic acids research, 2015 - academic.oup.com
In the plenitude of naturally occurring RNAs, circular RNAs (circRNAs) and their biological
role were underestimated for years. However, circRNAs are ubiquitous in all domains of life …
role were underestimated for years. However, circRNAs are ubiquitous in all domains of life …
Pervasive translation of circular RNAs driven by short IRES-like elements
Some circular RNAs (circRNAs) were found to be translated through IRES-driven
mechanism, however the scope and functions of circRNA translation are unclear because …
mechanism, however the scope and functions of circRNA translation are unclear because …
CircPro: an integrated tool for the identification of circRNAs with protein-coding potential
Circular RNAs (circRNAs), a novel class of endogenous RNAs, are widespread in eukaryotic
cells. Emerging roles in diverse biological processes suggest that circRNA is a promising …
cells. Emerging roles in diverse biological processes suggest that circRNA is a promising …
The biogenesis, functions, and challenges of circular RNAs
Covalently closed circular RNAs (circRNAs) are produced by precursor mRNA back-splicing
of exons of thousands of genes in eukaryotes. circRNAs are generally expressed at low …
of exons of thousands of genes in eukaryotes. circRNAs are generally expressed at low …
Comprehensive analysis of translation from overexpressed circular RNAs reveals pervasive translation from linear transcripts
Circular RNAs (circRNAs) encompass a widespread and conserved class of RNAs, which
are generated by back-splicing of downstream 5′ to upstream 3′ splice sites. CircRNAs …
are generated by back-splicing of downstream 5′ to upstream 3′ splice sites. CircRNAs …