Tuning the microenvironment to create functionally distinct mesenchymal stromal cell spheroids

VL Thai, DO Candelas, JK Leach - Annals of biomedical engineering, 2023 - Springer
Mesenchymal stromal cells (MSCs) are under investigation for wound healing and tissue
regeneration due to their potent secretome. Compared to monodisperse cells, MSC …

Multifactorial experimental design to optimize the anti-inflammatory and proangiogenic potential of mesenchymal stem cell spheroids

KC Murphy, J Whitehead, PC Falahee, D Zhou… - Stem Cells, 2017 - academic.oup.com
Mesenchymal stem cell therapies promote wound healing by manipulating the local
environment to enhance the function of host cells. Aggregation of mesenchymal stem cells …

Engineering fibrin hydrogels to promote the wound healing potential of mesenchymal stem cell spheroids

KC Murphy, J Whitehead, D Zhou, SS Ho, JK Leach - Acta biomaterialia, 2017 - Elsevier
Mesenchymal stem cells (MSCs) secrete endogenous factors such as vascular endothelial
growth factor (VEGF) and prostaglandin E2 (PGE 2) that promote angiogenesis, modulate …

Biomimetic culture strategies for the clinical expansion of mesenchymal stromal cells

KU Wong, A Zhang, B Akhavan… - … Science & Engineering, 2021 - ACS Publications
Mesenchymal stromal/stem cells (MSCs) typically require significant ex vivo expansion to
achieve the high cell numbers required for research and clinical applications. However …

Increased survival and function of mesenchymal stem cell spheroids entrapped in instructive alginate hydrogels

SS Ho, KC Murphy, BYK Binder… - Stem cells …, 2016 - academic.oup.com
Mesenchymal stem cell (MSC)-based therapies are under broad investigation for
applications in tissue repair but suffer from poor cell persistence and engraftment upon …

Structurally and mechanically tuned macroporous hydrogels for scalable mesenchymal stem cell–extracellular matrix spheroid production

S Yin, H Wu, Y Huang, C Lu, J Cui, Y Li, B Xue… - Proceedings of the …, 2024 - pnas.org
Mesenchymal stem cells (MSCs) are essential in regenerative medicine. However,
conventional expansion and harvesting methods often fail to maintain the essential …

Mesenchymal stromal cells as cell‐based therapeutics for wound healing

S Malhotra, MS Hu, CD Marshall, T Leavitt… - Stem cells …, 2016 - Wiley Online Library
Chronic wounds are a source of substantial morbidity for patients and are a major financial
burden for the healthcare system. There are no current therapies that reliably improve …

Strategies to capitalize on cell spheroid therapeutic potential for tissue repair and disease modeling

KH Griffin, SW Fok, J Kent Leach - NPJ Regenerative Medicine, 2022 - nature.com
Cell therapies offer a tailorable, personalized treatment for use in tissue engineering to
address defects arising from trauma, inefficient wound repair, or congenital malformation …

[HTML][HTML] Production and administration of therapeutic mesenchymal stem/stromal cell (MSC) spheroids primed in 3-D cultures under xeno-free conditions

JH Ylostalo, N Bazhanov… - Journal of visualized …, 2017 - ncbi.nlm.nih.gov
Mesenchymal stem/stromal cells (MSCs) hold great promise in bioengineering and
regenerative medicine. MSCs can be isolated from multiple adult tissues via their strong …

Engineered Cell‐Secreted Extracellular Matrix Modulates Cell Spheroid Mechanosensing and Amplifies Their Response to Inductive Cues for the Formation of …

T Gonzalez‐Fernandez, AJ Tenorio… - Advanced …, 2022 - Wiley Online Library
The clinical translation of mesenchymal stromal cell (MSC)‐based therapies remains
challenging due to rapid cell death and poor control over cell behavior. Compared to …