Light-sheet fluorescence microscopy is an invaluable tool for four-dimensional biological
imaging of multicellular systems due to the rapid volumetric imaging and minimal …

Live‐cell imaging of biological structures at high resolution poses challenges in the
microscope throughput regarding area and speed. For this reason, different parallelisation …

Diffraction-limited lens-based optical microscopy fails to discern fluorescent features closer
than∼ 200 nm. All super-resolution microscopy (nanoscopy) approaches that fundamentally …

Up to now, all demonstrations of reversible saturable optical fluorescence transitions
(RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly …

The super-resolution microscopy called RESOLFT relying on fluorophore switching between
longlived states, stands out by its coordinate-targeted sequential sample interrogation using …

We present a plane-scanning RESOLFT [reversible saturable/switchable optical
(fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the …

Two long-standing problems for superresolution (SR) fluorescence microscopy are high
illumination intensity and long acquisition time, which significantly hamper its application for …

Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy
methods, the STED/RESOLFT super-resolution family has recently been expanded to image …

Fluorescence superresolution (SR) microscopy, or fluorescence nanoscopy, provides
nanometer scale detail of cellular structures and allows for imaging of biological processes …

Superresolution fluorescence microscopy overcomes the diffraction resolution barrier and
allows the molecular intricacies of life to be revealed with greatly enhanced detail. However …