Novel pan-genomic analysis approach in target selection for multiplex PCR identification and detection of Burkholderia pseudomallei, Burkholderia thailandensis, and …

CC Ho, CCY Lau, P Martelli, SY Chan… - Journal of clinical …, 2011 - Am Soc Microbiol
Burkholderia pseudomallei, Burkholderia thailandensis, and the Burkholderia cepacia
complex differ greatly in pathogenicity and epidemiology. Yet, they are occasionally …

Development and Validation of Burkholderia pseudomallei-Specific Real-Time PCR Assays for Clinical, Environmental or Forensic Detection Applications

EP Price, JL Dale, JM Cook, DS Sarovich… - PLoS …, 2012 - journals.plos.org
The bacterium Burkholderia pseudomallei causes melioidosis, a rare but serious illness that
can be fatal if untreated or misdiagnosed. Species-specific PCR assays provide a …

Single gene target bacterial identification: groEL gene sequencing for discriminating clinical isolates of Burkholderia pseudomallei and Burkholderia thailandensis

PCY Woo, GKS Woo, SKP Lau, SSY Wong… - … and infectious disease, 2002 - Elsevier
Proper identification of Burkholderia pseudomallei and Burkholderia thailandensis is crucial
in guiding clinical management of patients with suspected melioidosis, as more than 99% of …

Identification of Burkholderia pseudomallei and Related Bacteria by Multiple-Locus Sequence Typing-Derived PCR and Real-Time PCR

P Wattiau, M Van Hessche, H Neubauer… - Journal of clinical …, 2007 - Am Soc Microbiol
Close relatedness and genomic plasticity characterizing the high-threat pathogens
Burkholderia pseudomallei and Burkholderia mallei render the molecular diagnosis of these …

DNA–DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing

V Ramisse, J Balandreau, F Thibault… - … of systematic and …, 2003 - microbiologyresearch.org
The present study was aimed at simplifying procedures to delineate species and identify
isolates based on DNA–DNA reassociation. DNA macro-arrays harbouring genomic DNA of …

Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species

EP Price, B MacHunter, BG Spratt… - Journal of medical …, 2016 - microbiologyresearch.org
The Burkholderia pseudomallei multilocus sequence typing (MLST) database
(http://pubmlst. org/bpseudomallei/) contains the largest global sequence repository for B …

Evaluation of species-specific recA-based PCR tests for genomovar level identification within the Burkholderia cepacia complex

K Vermis, T Coenye… - Journal of medical …, 2002 - microbiologyresearch.org
The Burkholderia cepacia complex presently comprises nine genomovars: B. cepacia
(genomovar I), B. multivorans (genomovar II), B. cepacia genomovar III, B. stabilis …

Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

I Janse, RA Hamidjaja, ACA Hendriks… - BMC Infectious …, 2013 - Springer
Background Burkholderia mallei and B. pseudomallei are two closely related species of
highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic …

Evaluation of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for identification of clinical and environmental …

H Wang, YL Chen, SH Teng, ZP Xu, YC Xu… - Frontiers in …, 2016 - frontiersin.org
Burkholderia pseudomallei is not represented in the current version of Bruker Biotyper
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) …

Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR

EP Price, DS Sarovich, JR Webb, JL Ginther, M Mayo… - PLoS …, 2013 - journals.plos.org
Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia
cepacia complex (Bcc), a group of genetically related organisms that are associated with …