A key to totipotency: Wuschel‐like homeobox 2a unlocks embryogenic culture response in maize (Zea mays L.)

FL McFarland, R Collier, N Walter… - Plant Biotechnology …, 2023 - Wiley Online Library
The ability of plant somatic cells to dedifferentiate, form somatic embryos and regenerate
whole plants in vitro has been harnessed for both clonal propagation and as a key …

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

K Lowe, E Wu, N Wang, G Hoerster, C Hastings… - The Plant …, 2016 - academic.oup.com
While transformation of the major monocot crops is currently possible, the process typically
remains confined to one or two genotypes per species, often with poor agronomics, and …

Maize Transformation Using the Morphogenic Genes Baby Boom and Wuschel2

T Jones, K Lowe, G Hoerster, A Anand, E Wu… - … Plants: Methods and …, 2019 - Springer
Despite the fact that maize transformation has been available for over 25 years, the
technology has remained too specialized, labor-intensive, and inefficient to be useful for the …

The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula

VE Tvorogova, YA Fedorova… - Plant Cell, Tissue and …, 2019 - Springer
Somatic embryogenesis is widely used in plant biotechnology, but regulatory networks
underlying this process are not fully understood. This process is regulated by many protein …

Use of non-integrating Zm-Wus2 vectors to enhance maize transformation: Non-integrating WUS2 enhances transformation

G Hoerster, N Wang, L Ryan, E Wu, A Anand… - In Vitro Cellular & …, 2020 - Springer
The use of Baby boom (Bbm) and Wuschel2 (Wus2) has made maize transformation more
efficient across an increasingly wide range of inbreds. However, the benefits have come with …

Coexpression of WUSCHEL related homeobox (WOX) 2 with WOX8 or WOX9 promotes regeneration from leaf segments and free cells in Nicotiana tabacum L.

M Kyo, K Maida, Y Nishioka, K Matsui - Plant Biotechnology, 2018 - jstage.jst.go.jp
To examine the effect of the ectopic expression of three Arabidopsis genes, including WOX2,
WOX8 and WOX9, on the regenerative competency of tissues and cells cultured in vitro, we …

Rapid genotype “independent” Zea mays L. (maize) transformation via direct somatic embryogenesis

K Lowe, M La Rota, G Hoerster, C Hastings… - In Vitro Cellular & …, 2018 - Springer
Constitutive expression of the Zea mays L.(maize) morphogenic transcription factors Baby
Boom (Bbm) and Wuschel2 (Wus2) in maize can not only greatly increase transformation …

Expression dynamics of WOX genes mark cell fate decisions during early embryonic patterning in Arabidopsis thaliana

A Haecker, R Groß-Hardt, B Geiges, A Sarkar… - 2004 - journals.biologists.com
During embryonic pattern formation, the main body axes are established and cells of
different developmental fates are specified from a single-cell zygote. Despite the …

Ectopic expression of the maize homeobox genes ZmHox1a or ZmHox1b causes pleiotropic alterations in the vegetative and floral development of transgenic tobacco.

B Uberlacker, B Klinge, W Werr - The Plant Cell, 1996 - academic.oup.com
Abstract The ZmHox1a and ZmHox1b (for Zea mays homeobox) genes map on
chromosomes 8 and 6, respectively. Both homeobox genes encode proteins that show 91 …

An efficient gene excision system in maize

N Wang, M Arling, G Hoerster, L Ryan, E Wu… - Frontiers in plant …, 2020 - frontiersin.org
Use of the morphogenic genes Baby Boom (Bbm) and Wuschel2 (Wus2), along with new
ternary constructs, has increased the genotype range and the type of explants that can be …