Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

E Bauda, B Gallet, J Moravcova, G Effantin… - Nature …, 2024 - nature.com
Bacterial spores owe their incredible resistance capacities to molecular structures that
protect the cell content from external aggressions. Among the determinants of resistance are …

The molecular architecture of engulfment during Bacillus subtilis sporulation

K Khanna, J Lopez-Garrido, Z Zhao, R Watanabe… - Elife, 2019 - elifesciences.org
The study of bacterial cell biology is limited by difficulties in visualizing cellular structures at
high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis …

[HTML][HTML] The high-resolution architecture and structural dynamics of Bacillus spores

M Plomp, TJ Leighton, KE Wheeler, AJ Malkin - Biophysical journal, 2005 - cell.com
The capability to image single microbial cell surfaces at nanometer scale under native
conditions would profoundly impact mechanistic and structural studies of pathogenesis …

Molecular architecture of the assembly of Bacillus spore coat protein GerQ revealed by cryo-EM

Y Cheng, MAB Kreutzberger, J Han… - Nature …, 2024 - nature.com
Protein filaments are ubiquitous in nature and have diverse biological functions. Cryo-
electron microscopy (cryo-EM) enables the determination of atomic structures, even from …

Structure and assembly of the bacterial endospore coat

AO Henriques, CP Moran Jr - Methods, 2000 - Elsevier
Many biological processes are mediated through the action of multiprotein complexes, often
assembled at specific cellular locations. Bacterial endospores for example, are encased in a …

Bacillus atrophaeus Outer Spore Coat Assembly and Ultrastructure

M Plomp, TJ Leighton, KE Wheeler, ME Pitesky… - Langmuir, 2005 - ACS Publications
Our previous atomic force microscopy (AFM) studies successfully visualized native Bacillus
atrophaeus spore coat ultrastructure and surface morphology. We have shown that the outer …

Near-atomic resolution cryoelectron microscopy structure of the 30-fold homooligomeric SpoIIIAG channel essential to spore formation in Bacillus subtilis

N Zeytuni, C Hong, KA Flanagan… - Proceedings of the …, 2017 - National Acad Sciences
Bacterial sporulation allows starving cells to differentiate into metabolically dormant spores
that can survive extreme conditions. Following asymmetric division, the mother cell engulfs …

[HTML][HTML] Dormant Bacillus spores protect their DNA in crystalline nucleoids against environmental stress

C Dittmann, HM Han, M Grabenbauer… - Journal of Structural …, 2015 - Elsevier
Bacterial spores of the genera Bacillus and Clostridium are extremely resistant against
desiccation, heat and radiation and involved in the spread and pathogenicity of health …

Assembly and genetics of spore protective structures

H Takamatsu, K Watabe - Cellular and Molecular Life Sciences CMLS, 2002 - Springer
The sporulation program in Bacillus subtilis ends in the formation of a highly resistant
endospore that can withstand extremes of heat, mechanical disruption, ultraviolet irradiation …

Analysis of the role of bacterial endospore cortex structure in resistance properties and demonstration of its conservation amongst species

A Atrih, SJ Foster - Journal of applied microbiology, 2001 - academic.oup.com
Aims: The aim of this work was to compare the chemical structure of the spore cortex of a
range of species, and to determine any correlation between cortex structure and spore …