High performance liquid chromatography (HPLC) with fluorescence detection for quantification of steroids in clinical, pharmaceutical, and environmental samples: A …

F Hameedat, S Hawamdeh, S Alnabulsi, A Zayed - Molecules, 2022 - mdpi.com
Steroids are compounds widely available in nature and synthesized for therapeutic and
medical purposes. Although several analytical techniques are available for the quantification …

Derivatization of neutral steroids to enhance their detection characteristics in liquid chromatography–mass spectrometry

T Higashi, K Shimada - Analytical and bioanalytical chemistry, 2004 - Springer
This review article underlines the detection-oriented derivatization of neutral steroids in
liquid chromatography–mass spectrometry (LC–MS). Steroids have strong biological activity …

Quantitative-profiling method of serum steroid hormones by hydroxylamine-derivatization HPLC–MS

Q Liu, Q Chi, RT Fan, HD Tian, X Wang - Natural Products and …, 2019 - Springer
A sensitive and rapid high performance liquid chromatography–mass spectrometry (HPLC–
MS) method was developed and validated for simultaneous quantification of ten steroid …

Chemical derivatization for enhancing sensitivity during LC/ESI–MS/MS quantification of steroids in biological samples: a review

T Higashi, S Ogawa - The Journal of Steroid Biochemistry and Molecular …, 2016 - Elsevier
Sensitive and specific methods for the detection, characterization and quantification of
endogenous steroids in body fluids or tissues are necessary for the diagnosis, pathological …

[HTML][HTML] Straightforward quantification of endogenous steroids with liquid chromatography–tandem mass spectrometry: Comparing calibration approaches

G Visconti, M De Figueiredo, O Salamin… - … of Chromatography B, 2023 - Elsevier
Different calibration strategies are used in liquid chromatography hyphenated to mass
spectrometry (LC-MS) bioanalysis. Currently, the surrogate matrix and surrogate analyte …

Simultaneous analysis of free and sulfated steroids by liquid chromatography/mass spectrometry with selective mass spectrometric scan modes and polarity switching

SH Lee, N Lee, Y Hong, BC Chung… - Analytical chemistry, 2016 - ACS Publications
Sulfated steroids can act as a latent form of active free steroids, coexisting with them in
biological specimens. To evaluate the metabolic significance of free and sulfated steroid …

Gas chromatography and high-performance liquid chromatography of natural steroids

K Shimada, K Mitamura, T Higashi - Journal of Chromatography A, 2001 - Elsevier
This review article underlines the importance of gas chromatography (GC), high-
performance liquid chromatography (HPLC) and their hyphenated techniques using mass …

Derivatization of steroids in biological samples for GC–MS and LC–MS analyses

J Marcos, OJ Pozo - Bioanalysis, 2015 - Taylor & Francis
The determination of steroids in biological samples is essential in different areas of
knowledge. MS combined with either GC or LC is considered the best analytical technique …

Novel liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for measuring steroids

BG Keevil - Best practice & research Clinical endocrinology & …, 2013 - Elsevier
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming
the method of choice for steroid hormone measurements due to small sample volumes, fast …

Parallel derivatization strategy coupled with liquid chromatography-mass spectrometry for broad coverage of steroid hormones

Q Qin, D Feng, C Hu, B Wang, M Chang, X Liu… - … of Chromatography a, 2020 - Elsevier
Steroid hormones are a type of crucial substances that mediate numerous vital physiological
functions. The comprehensive detection of steroid hormones can help understand the …