Strategies, challenges and opportunities of enzyme immobilization on porous silicon for biosensing applications

R Fopase, S Paramasivam, P Kale… - Journal of Environmental …, 2020 - Elsevier
The use of porous silicon (PS) for biosensing applications is now emerging as it offers
desired properties such as better surface-volume ratio, tunable pore size, ease of …

A review on porous silicon based electrochemical biosensors: Beyond surface area enhancement factor

C RoyChaudhuri - Sensors and Actuators B: Chemical, 2015 - Elsevier
The use of porous silicon (PS) as an electrochemical biosensor has been reviewed. For
electrochemical biosensing, the enhanced surface area is not the only advantage of using …

Porous silicon biosensor: Current status

S Dhanekar, S Jain - Biosensors and bioelectronics, 2013 - Elsevier
Biosensing technologies cater to modern day diagnostics and point of care multi-specialty
clinics, hospitals and laboratories. Biosensors aggregate the sensitivity of detection …

Porous silicon chemical sensors and biosensors: A review

FA Harraz - Sensors and Actuators B: Chemical, 2014 - Elsevier
The use of porous silicon (PSi) as a sensor for detection of various analytes is reviewed. The
optical or electrical properties of PSi are key sensing parameters that have been used in …

Enzyme immobilisation on planar and porous silicon substrates for biosensor applications

M Thust, MJ Schöning, P Schroth, Ü Malkoc… - Journal of Molecular …, 1999 - Elsevier
Two methods for the immobilisation of enzymes on silicon-based so-called electrolyte–
insulator–semiconductor (EIS) structures are suggested. These EIS structures are used as a …

Immobilization of enzymes on porous silicas–benefits and challenges

M Hartmann, X Kostrov - Chemical Society Reviews, 2013 - pubs.rsc.org
Porous silica materials have extensively been used for the immobilization of enzymes
aiming at their use as biocatalysts or biosensors. This tutorial review will discuss the benefits …

Comparison of techniques for enzyme immobilization on silicon supports

A Subramanian, SJ Kennel, PI Oden… - Enzyme and Microbial …, 1999 - Elsevier
Enzyme immobilization onto silicon substrates has been investigated by five different
coupling procedures. The methods included covalent coupling either through a metal link …

Quantatitive assessment of enzyme immobilization capacity in porous silicon

LA DeLouise, BL Miller - Analytical chemistry, 2004 - ACS Publications
Immobilized enzyme systems are important in a broad range of applications, from biological
sensing to the industrial-scale biocatalytic synthesis of chiral products. We demonstrate the …

Applications of microstructured porous silicon as a biocatalytic surface

M Bengtsson, S Ekström, J Drott, A Collins… - … status solidi (a), 2000 - Wiley Online Library
The use of porous silicon (PS) as an efficient surface enlarging carrier matrix for immobilised
enzymes is demonstrated. An optimal porous matrix in p‐type silicon (10–20 Ωcm) with …

Enhanced enzyme activity in silicon integrated enzyme reactors utilizing porous silicon as the coupling matrix

T Laurell, J Drott, L Rosengren, K Lindström - Sensors and Actuators B …, 1996 - Elsevier
The performance of porous silicon as a coupling matrix for silicon integrated enzyme
reactors has been investigated. A porous silicon layer in a water is expected to yield an …