Three sequences in the vicinity of poly (A) addition sites are conserved among vertebrate
mRNAs. We analyze the effects of single base changes in each position of AAUAAA and in …

The hexanucleotide AAUAAA has been demonstrated to be part of the signal for cleavage
and polyadenylation at appropriate sites on eucaryotic mRNA precursors. Since this …

To study the mechanism and factors required to form the 3'ends of polyadenylated mRNAs,
we have fractionated HeLa cell nuclear extracts carrying out the normally coupled cleavage …

To determine whether a specific nucleotide sequence is required to direct polyadenylation of
a simian virus 40 early pre-mRNA in a soluble HeLa whole-cell lysate, we constructed a …

An SP6 RNA containing the adenovirus 5 L3 poly (A) site is processed efficiently in a HeLa
cell nuclear extract to generate correct 3'termini. Accurate 3'processing has also been …

A critical step in mRNA biogenesis is the generation of the mRNA 3′ end through an
endonucleolytic cleavage of the primary transcript followed by the addition of a …

From the date of its discovery in 1970, the 3'poly (A) tail of eukaryotic mRNA has taken on
the character of a structure in search of a function. Candidate functions favored in those far …

Polyadenylation in the nucleus 1 and in a cell-free nuclear extract 2 immediately follows a
cleavage reaction in which a phosphodiester bond in the mRNA precursor is broken (Fig. 1) …

Biological polyadenylation, first recognized as an enzymatic activity, remained an orphan
enzyme until poly A sequences were found on the 3′ ends of eukaryotic mRNAs. Their …

A poly (A) tail is found at the 3 end of nearly every fully processed eukaryotic mRNA and has
been suggested to influence virtually all aspects of mRNA metabolism. Its proposed …