A rational design for improving the trypsin resistance of aflatoxin-detoxifizyme (ADTZ) based on molecular structure evaluation
Y Qiu, X Wu, C Xie, Y Hu, D Liu, Y Ma, D Yao - Enzyme and Microbial …, 2016 - Elsevier
The resistance of feed enzymes against proteases is crucial in livestock farming. In this
study, the trypsin resistance of aflatoxin-detoxifizyme (ADTZ) is improved. ADTZ possesses …
study, the trypsin resistance of aflatoxin-detoxifizyme (ADTZ) is improved. ADTZ possesses …
Tuning thermostability and catalytic efficiency of aflatoxin-degrading enzyme by error-prone PCR
R Jia, S Tian, Z Yang, FA Sadiq, L Wang, S Lu… - Applied Microbiology …, 2023 - Springer
In our previous work, a recombinant aflatoxin-degrading enzyme derived from Myxococcus
fulvus (MADE) was reported. However, the low thermal stability of the enzyme had …
fulvus (MADE) was reported. However, the low thermal stability of the enzyme had …
Directed evolution of aflatoxin detoxifzyme in vitro by error-prone PCR
S Zhang, K Xing, Y Hu, C Xie, D Liu… - Sheng wu Gong Cheng …, 2011 - europepmc.org
The experiment was conducted by directed evolution strategy (error-prone PCR) to improve
the activity of aflatoxin detoxifzyme with the high-throughput horse radish peroxidas and …
the activity of aflatoxin detoxifzyme with the high-throughput horse radish peroxidas and …
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris
L Li, M Mei, J Wang, J Huang, X Zong… - Biotechnology …, 2024 - Wiley Online Library
In this study, three aflatoxin degrading enzyme genes, tv‐adtz, arm‐adtz and cu‐adtz, were
heterologously expressed in Pichia pastoris. The protein expression of the enzyme solution …
heterologously expressed in Pichia pastoris. The protein expression of the enzyme solution …
Recent technological advances in mechanism, toxicity, and food perspectives of enzyme-mediated aflatoxin degradation
Aflatoxins are carcinogenic secondary metabolites produced by Aspergillus section Flavi
that contaminates a wide variety of food and feed products and is responsible for serious …
that contaminates a wide variety of food and feed products and is responsible for serious …
Molecular docking studies and in vitro degradation of four aflatoxins (AFB1, AFB2, AFG1, and AFG2) by a recombinant laccase from Saccharomyces cerevisiae
Y Liu, H Mao, C Hu, T Tron, J Lin, J Wang… - Journal of food …, 2020 - Wiley Online Library
Here, molecular docking simulation was used to predict and compare interactions between
a recombinant Trametes sp. C30 laccase from Saccharomyces cerevisiae and four …
a recombinant Trametes sp. C30 laccase from Saccharomyces cerevisiae and four …
DypB peroxidase for aflatoxin removal: New insights into the toxin degradation process
Abstract Aflatoxin B 1 (AFB 1) is one of the most potent carcinogens and a widespread food
and feed contaminant. As for other toxins, many efforts are devoted to find efficient and …
and feed contaminant. As for other toxins, many efforts are devoted to find efficient and …
Crystal structures of Aflatoxin-oxidase from Armillariella tabescens reveal a dual activity enzyme
Abstract Aflatoxin-oxidase (AFO), a newly discovered oxidase isolated from Armillariella
tabescens, was reported to perform aflatoxin B1 (AFB1) detoxification through breaking the …
tabescens, was reported to perform aflatoxin B1 (AFB1) detoxification through breaking the …
[PDF][PDF] Cloning, expression, purification and characterization of an aflatoxin-converting enzyme from Armillaria tabescens
S Wen, M Guan, T Zhou, H Cao, C Xie… - Wei Sheng Wu Xue …, 2011 - researchgate.net
[Objective] Aflatoxin B1 (AFB1) is extremely mutagenic, toxic and a potent carcinogen both to
humans and livestock. Aflatoxin-oxidase (AFO) was an aflatoxin-converting enzyme …
humans and livestock. Aflatoxin-oxidase (AFO) was an aflatoxin-converting enzyme …
Activity enhancement of Trametes versicolor aflatoxin B1-degrading enzyme (TV-AFB1D) by molecular docking and site-directed mutagenesis techniques
Abstract Aflatoxin B 1 (AFB 1) is highly carcinogenic and teratogenic as a secondary
metabolite of Aspergillus flavus and Aspergillus parasiticus. Trametes versicolor aflatoxin B …
metabolite of Aspergillus flavus and Aspergillus parasiticus. Trametes versicolor aflatoxin B …