Whole-genome analyses: average nucleotide identity

DR Arahal - Methods in microbiology, 2014 - Elsevier
Average nucleotide identity (ANI) was proposed almost 10 years ago as a means to
compare genetic relatedness among prokaryotic strains. It was found that values around …

OrthoANI: an improved algorithm and software for calculating average nucleotide identity

I Lee, Y Ouk Kim, SC Park… - International journal of …, 2016 - microbiologyresearch.org
Species demarcation in Bacteria and Archaea is mainly based on overall genome
relatedness, which serves a framework for modern microbiology. Current practice for …

Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes

M Kim, HS Oh, SC Park, J Chun - International journal of …, 2014 - microbiologyresearch.org
Among available genome relatedness indices, average nucleotide identity (ANI) is one of
the most robust measurements of genomic relatedness between strains, and has great …

Improving phylogenies based on average nucleotide identity, incorporating saturation correction and nonparametric bootstrap support

S Gosselin, MS Fullmer, Y Feng… - Systematic …, 2022 - academic.oup.com
Whole-genome comparisons based on average nucleotide identities (ANI) and the genome-
to-genome distance calculator have risen to prominence in rapidly classifying prokaryotic …

A large-scale evaluation of algorithms to calculate average nucleotide identity

SH Yoon, S Ha, J Lim, S Kwon, J Chun - Antonie Van Leeuwenhoek, 2017 - Springer
Average nucleotide identity (ANI) is a category of computational analysis that can be used to
define species boundaries of Archaea and Bacteria. Calculating ANI usually involves the …

Genome-based taxonomic classification

B Paul, G Dixit, TS Murali, K Satyamoorthy - Genome, 2019 - cdnsciencepub.com
Bacterial populations are routinely characterized based on microscopic examination, colony
formation, and biochemical tests. However, in the recent past, bacterial identification …

The relationship of the whole genome sequence identity to DNA hybridization varies between genera of prokaryotes

X Li, Y Huang, WB Whitman - Antonie van Leeuwenhoek, 2015 - Springer
In the original proposal of Wayne et al.(Int J Syst Bacteriol 37: 463–464, 1987), two
measures of genetic relatedness were proposed to set the boundary for prokaryotic species …

Introducing EzAAI: a pipeline for high throughput calculations of prokaryotic average amino acid identity

D Kim, S Park, J Chun - Journal of Microbiology, 2021 - Springer
The average amino acid identity (AAI) is an index of pairwise genomic relatedness, and
multiple studies have proposed its application in prokaryotic taxonomy and related …

ANItools web: a web tool for fast genome comparison within multiple bacterial strains

N Han, Y Qiang, W Zhang - Database, 2016 - academic.oup.com
Background: Early classification of prokaryotes was based solely on phenotypic similarities,
but modern prokaryote characterization has been strongly influenced by advances in …

Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs

AF Auch, HP Klenk, M Göker - Standards in genomic sciences, 2010 - Springer
Abstract DNA-DNA hybridization (DDH) is a widely applied wet-lab technique to obtain an
estimate of the overall similarity between the genomes of two organisms. To base the …