[HTML][HTML] Double-stranded RNA uptake through topical application, mediates silencing of five CYP4 genes and suppresses insecticide resistance in Diaphorina citri

N Killiny, S Hajeri, S Tiwari, S Gowda, LL Stelinski - PloS one, 2014 - journals.plos.org
Silencing of genes through RNA interference (RNAi) in insects has gained momentum
during the past few years. RNAi has been used to cause insect mortality, inhibit insect …

Silencing of cytochrome P450 CYP6B6 gene of cotton bollworm (Helicoverpa armigera) by RNAi

X Zhang, X Liu, J Ma, J Zhao - Bulletin of entomological research, 2013 - cambridge.org
RNA interference (RNAi) induced through double-stranded RNA (dsRNA) has been used
widely to study gene function in insects. In this paper we demonstrate the efficacy of RNAi in …

Effect of RNAi targeting CYP6CY3 on the growth, development and insecticide susceptibility of Aphis gossypii by using nanocarrier-based transdermal dsRNA …

W Linyu, Z Lianjun, L Ning, G Xiwu… - Pesticide Biochemistry and …, 2021 - Elsevier
RNA interference (RNAi) has been proved to be a viable method for agricultural pest control.
Due to the limited uptake of dsRNA in hemiptera insects, this study used nanocarrier SPc …

RNA interference-mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin

MAM Bautista, T Miyata, K Miura, T Tanaka - Insect biochemistry and …, 2009 - Elsevier
We have previously reported that a cytochrome P450, CYP6BG1, from Plutella xylostella
was found to be overexpressed in 4th instars of a permethrin resistant strain and inducible in …

Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

X Yu, S Gowda, N Killiny - Pest Management Science, 2017 - Wiley Online Library
BACKGROUND Asian citrus psyllid, Diaphorina citri Kuwayama, is the most important
economic pest of citrus because it transmits Candidatus Liberibacter asiaticus (C Las), the …

Double-stranded RNA oral delivery methods to induce RNA interference in phloem and plant-sap-feeding hemipteran insects

SKB Ghosh, WB Hunter, AL Park… - JoVE (Journal of …, 2018 - jove.com
Phloem and plant sap feeding insects invade the integrity of crops and fruits to retrieve
nutrients, in the process damaging food crops. Hemipteran insects account for a number of …

Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis

AMW Cooper, H Song, Z Yu, M Biondi… - Pest management …, 2021 - Wiley Online Library
BACKGROUND Targeting insect‐specific genes through post‐transcriptional gene silencing
with RNA interference (RNAi) is a new strategy for insect pest management. However …

Strategies for enhancing the efficiency of RNA interference in insects

K Silver, AMW Cooper, KY Zhu - Pest Management Science, 2021 - Wiley Online Library
Low RNA interference (RNAi) efficiency in many insect pests has significantly prevented its
widespread application for insect pest management. This article provides a comprehensive …

Screening of lethal genes for feeding RNAi by leaf disc-mediated systematic delivery of dsRNA in Tetranychus urticae

DH Kwon, JH Park, SH Lee - Pesticide Biochemistry and Physiology, 2013 - Elsevier
To identify genes that kill Tetranychus urticae when knocked down via RNA interference
(RNAi), several lethal genes were screened by the systemic delivery of dsRNA via leaf disc …

Delivery of dsRNA through topical feeding for RNA interference in the citrus sap piercing‐sucking hemipteran, Diaphorina citri

N Killiny, A Kishk - Archives of Insect Biochemistry and …, 2017 - Wiley Online Library
RNA interference (RNAi) is a powerful means to study functional genomics in insects. The
delivery of dsRNA is a challenging step in the development of RNAi assay. Here, we …