A review of electrophoretic separations in temperature-responsive Pluronic thermal gels

CL Ward, MA Cornejo, SHP Thanthri, TH Linz - Analytica Chimica Acta, 2023 - Elsevier
Gel electrophoresis is a ubiquitous bioanalytical technique used in research laboratories to
validate protein and nucleic acid samples. Polyacrylamide and agarose have been the gold …

Characterizing the impact of thermal gels on isotachophoresis in microfluidic devices

CL Ward, TH Linz - Electrophoresis, 2020 - Wiley Online Library
Thermally reversible Pluronic gels have been employed as separation matrices in
microfluidic devices in the analysis of biological macromolecules. The phase of these gels …

Heat dissipation in slab gel electrophoresis: The effect of embedded TiO2 nanoparticles on the thermal profiles

SH Sajjadi, EK Goharshadi, H Ahmadzadeh - Journal of Chromatography B, 2019 - Elsevier
Despite the fast development of novel and high-resolution electrophoresis techniques such
as capillary-based methods and microfluidic devices, the slab gel electrophoresis is still a …

Temperature-responsive polymers for liquid-phase separations

H Kanazawa - Analytical and bioanalytical chemistry, 2004 - Springer
Fig. 1 Temperature response on changing the property of a silica surface in temperature-
responsive chromatography bicity and charge density for separation of bioactive compounds …

Harnessing Joule heating in microfluidic thermal gel electrophoresis to create reversible barriers for cell enrichment

MA Cornejo, TH Linz - Electrophoresis, 2021 - Wiley Online Library
Gel electrophoresis is a ubiquitous bioanalytical technique used to characterize the
components of cell lysates. However, analyses of bulk lysates sacrifice detection sensitivity …

Gels in microscale electrophoresis

LA Holland, LD Casto-Boggess - Annual Review of Analytical …, 2023 - annualreviews.org
Gel matrices are fundamental to electrophoresis analyses of biopolymers in microscale
channels. Both capillary gel and microchannel gel electrophoresis systems have produced …

Diffusion and Concentration of Molecular Probes in Thermoresponsive Poly(N-isopropylacrylamide) Hydrogels:  Effect of the Volume Phase Transition

W Zhang, I Gaberman, M Ciszkowska - Analytical chemistry, 2002 - ACS Publications
Poly (N-isopropylacrylamide), NIPA, thermoresponsive hydrogels with well-defined
concentrations of an electroactive probe, 1, 1 '-ferrocenedimethanol, Fc (MeOH) 2, were …

Effect of the Volume Phase Transition on Diffusion and Concentration of Molecular Species in Temperature‐Responsive Gels: Electroanalytical Studies

W Zhang, I Gaberman… - … : An International Journal …, 2003 - Wiley Online Library
Abstract Effect of the volume phase transition on the diffusion and concentration of molecular
probes was studied in temperature‐responsive polymeric hydrogels. Results were …

Characterization of a reversible thermoresponsive gel and its application to oligonucleotide separation

J Zhang, M Gassmann, X Chen, C Burger… - …, 2007 - ACS Publications
Pluronic mixtures of F87 (E61P40E61) and F127 (E99P69E99), with E and P being poly
(ethylene oxide) and poly (propylene oxide), respectively, have been used as effective …

Reversible thermo-responsive sieving matrix for oligonucleotide separation

J Zhang, M Gassmann, W He, F Wan, B Chu - Lab on a Chip, 2006 - pubs.rsc.org
A reversible thermo-responsive gel system, consisting of Pluronic copolymer mixture of F87
and F127, has been used to successfully carry out the separation of oligonucleotides, for the …