Regulatory mechanisms for amylolytic gene expression in the koji mold Aspergillus oryzae

K Gomi - Bioscience, Biotechnology, and Biochemistry, 2019 - academic.oup.com
The koji mold Aspergillus oryzae has been used in traditional Japanese food and beverage
fermentation for over a thousand years. Amylolytic enzymes are important in sake …

Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae

K Gomi, T Akeno, T Minetoki, K Ozeki… - Bioscience …, 2000 - academic.oup.com
A gene, designated amyR, coding for a transcriptional activator involved in amylolytic gene
expression has been cloned from Aspergillus oryzae by screening for a clone that enabled …

Crucial role of the intracellular α-glucosidase MalT in the activation of the transcription factor AmyR essential for amylolytic gene expression in Aspergillus oryzae

T Ichikawa, M Tanaka, T Watanabe… - Bioscience …, 2021 - academic.oup.com
We examined the role of the intracellular α-glucosidase gene malT, which is part of the
maltose-utilizing cluster (MAL cluster) together with malR and malP, in amylolytic gene …

Distinct mechanism of activation of two transcription factors, AmyR and MalR, involved in amylolytic enzyme production in Aspergillus oryzae

K Suzuki, M Tanaka, Y Konno, T Ichikawa… - Applied microbiology …, 2015 - Springer
The production of amylolytic enzymes in Aspergillus oryzae is induced in the presence of
starch or maltose, and two Zn 2 Cys 6-type transcription factors, AmyR and MalR, are …

Regulation of the glucoamylase-encoding gene (glaB), expressed in solid-state culture (koji) of Aspergillus oryzae

H Ishida, Y Hata, E Ichikawa, A Kawato… - Journal of fermentation …, 1998 - Elsevier
Aspergillus oryzae has two glucoamylase-encoding genes, glaA and glaB, the patterns of
expression of which are different. Expression of the glaB gene is marked in solid-state …

Contribution Ratios of amyA, amyB, amyC Genes to High-Level α-Amylase Expression in Aspergillus oryzae

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2012 - academic.oup.com
Aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain
RIB40 has three α-amylase genes (amyA, amyB, and amyC). However, there is no …

Induction and Repression of Hydrolase Genes in Aspergillus oryzae

M Tanaka, K Gomi - Frontiers in Microbiology, 2021 - frontiersin.org
The filamentous fungus Aspergillus oryzae, also known as yellow koji mold, produces high
levels of hydrolases such as amylolytic and proteolytic enzymes. This property of producing …

Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2009 - academic.oup.com
Aspergillus oryzae RIB40 has three α-amylase genes (amyA, amyB, and amyC), and
secretes α-amylase abundantly. However, large amounts of endogenous secretory proteins …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

Inducer-dependent nuclear localization of a Zn (II) 2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans

T Makita, Y Katsuyama, S Tani, H Suzuki… - Bioscience …, 2009 - jstage.jst.go.jp
AmyR is a Zn (II) 2Cys6 transcriptional activator that regulates expression of the amylolytic
genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A …