High affinity truncated DNA aptamers for the development of fluorescence based progesterone biosensors

HA Alhadrami, R Chinnappan, S Eissa… - Analytical …, 2017 - Elsevier
Aptamers have shown a number of potential applications in sensing and therapeutic due to
the high affinity and specificity towards their target molecules. Not all the nucleotides in the …

Aptamer-based label-free impedimetric biosensor for detection of progesterone

G Contreras Jiménez, S Eissa, A Ng… - Analytical …, 2015 - ACS Publications
Rising progesterone (P4) levels in humans due to its overconsumption through hormonal
therapy, food products, or drinking water can lead to many negative health effects. Thus, the …

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

G Du, D Zhang, B Xia, L Xu, S Wu, S Zhan, X Ni… - Microchimica Acta, 2016 - Springer
The authors describe an aptasensor for the detection of the gonadal hormone progesterone
(P4) in aqueous solution. Gold nanoparticles (AuNPs) were coated with a P4-specific …

A simple FRET-based turn-on fluorescent aptasensor for 17β-estradiol determination in environmental water, urine and milk samples

G Zhang, T Li, J Zhang, A Chen - Sensors and Actuators B: Chemical, 2018 - Elsevier
A competitive fluorescent aptasensor was constructed based on fluorescence resonance
energy transfer (FRET) for determination of 17β-estradiol (E2). Initially, carboxyfluorescein …

Lateral flow aptasensor for progesterone: Competitive target recognition and displacement of short complementary sequences

MN Alnajrani, OA Alsager - Analytical biochemistry, 2019 - Elsevier
There is a pressing need for simple and accurate analytical tools to assess the level of EDCs
in environmental samples. In this work, a simple and highly sensitive competitive lateral flow …

Fluorescent aptasensor for 17β-estradiol determination based on gold nanoparticles quenching the fluorescence of Rhodamine B

X Ni, B Xia, L Wang, J Ye, G Du, H Feng, X Zhou… - Analytical …, 2017 - Elsevier
In this paper, we developed a fluorescent aptasensor for 17β-estradiol (E2) determination in
aqueous solution using label-free E2-specific aptamer, gold nanoparticles (AuNPs) and …

Colorimetric aptasensor for progesterone detection based on surfactant-induced aggregation of gold nanoparticles

G Du, L Wang, D Zhang, X Ni, X Zhou, H Xu, L Xu… - Analytical …, 2016 - Elsevier
This paper proposes an aptasensor for progesterone (P4) detection in human serum and
urine based on the aggregating behavior of gold nanoparticles (AuNPs) controlled by the …

Aptamers biosensors for pharmaceutical compounds

S Tombelli, M Mascini - Combinatorial Chemistry & High …, 2010 - ingentaconnect.com
Aptamers are single stranded DNA or RNA ligands which can be selected for different
targets starting from a huge library of molecules containing randomly created sequences …

Aptatope mapping of the binding site of a progesterone aptamer on the steroid ring structure

V Skouridou, T Schubert, AS Bashammakh… - Analytical …, 2017 - Elsevier
In this work we report the mapping of the binding site of the only progesterone aptamer
published to date, in an approach referred to as aptatope mapping. By linking the binding …

Aptamers targeting different functional groups of 17β-estradiol

K Vanschoenbeek, J Vanbrabant… - The Journal of Steroid …, 2015 - Elsevier
Aptamers, short synthetic ssDNA or RNA molecules with a specific three-dimensional
structure, are promising recognition elements in biosensor technology. In vitro generation of …