For Aspergillus flavus, a pathogen of considerable economic and health concern, successful
gene knockout work for more than a decade has relied nearly exclusively on using …

Aspergillus, a genus of filamentous fungi, is extensively distributed in nature and plays
crucial roles in the decomposition of organic materials as an important environmental …

Objectives To develop a genome editing method using the CRISPR/Cas9 system in
Aspergillus oryzae, the industrial filamentous fungus used in Japanese traditional …

CRISPR-Cas9 technologies are revolutionizing fungal gene editing. Here we show that
survival of specific Cas9/sgRNA mediated DNA double strand breaks (DSBs) depends on …

As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been
applied in filamentous fungi to improve the efficiency of genome alteration. However, the …

Aspergillus niger and other filamentous fungi are widely used in industry, but efficient
genetic engineering of these hosts remains nascent. For example, while molecular genetic …

Aspergillus niger is an important industrial producer of enzymes due to its high capacity for
producing exocellular secretory proteins. The CRISPR/Cas9 system has been developed as …

Aspergillus fumigatus is a saprophytic fungal pathogen that is the cause of more than
300,000 life-threatening infections annually. Our understanding of pathogenesis and factors …

The number of fully sequenced fungal genomes is rapidly increasing. Since genetic tools
are poorly developed for most filamentous fungi, it is currently difficult to employ genetic …

Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing
multiple gene alterations in filamentous fungi, whereas traditional methods are time …