Natural transformation as a tool in Acinetobacter baylyi: Evolution by amplification of gene copy number

I Pardo, SR Bedore, MP Tumen-Velasquez… - Methods in …, 2023 - Elsevier
For many years, the natural competency of Acinetobacter baylyi ADP1 facilitated studies of
bacterial metabolism, biochemistry, and physiology. With the advent of synthetic biology …

OPPORTUNITIES FOR GENETIC INVESTIGATION AFFORDED BY ACINETOBACTER BAYLYI, A NUTRITIONALLY VERSATILE BACTERIAL SPECIES THAT IS …

DM Young, D Parke, LN Ornston - Annu. Rev. Microbiol., 2005 - annualreviews.org
The genetic and physiological properties of Acinetobacter baylyi strain ADP1 make it an
inviting subject for investigation of the properties underlying its nutritional versatility. The …

Rapid Evolution of Diminished Transformability in Acinetobacter baylyi

JM Bacher, D Metzgar… - Journal of bacteriology, 2006 - Am Soc Microbiol
The reason for genetic exchange remains a crucial question in evolutionary biology.
Acinetobacter baylyi strain ADP1 is a highly competent and recombinogenic bacterium. We …

Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution

BA Renda, A Dasgupta, D Leon… - Journal of bacteriology, 2015 - Am Soc Microbiol
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …

Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysis

SR Bedore, EL Neidle, I Pardo, J Luo, AC Baugh… - Methods in …, 2023 - Elsevier
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …

Acinetobacter baylyi ADP1: Transforming the choice of model organism

KT Elliott, EL Neidle - IUBMB life, 2011 - Wiley Online Library
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology
studies that address a broad range of questions. Recently, the rapid accumulation of data …

Expression vectors for Acinetobacter baylyi ADP1

CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …

Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering

D Metzgar, JM Bacher, V Pezo, J Reader… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …

A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1

V De Berardinis, D Vallenet, V Castelli… - Molecular systems …, 2008 - embopress.org
We have constructed a collection of single‐gene deletion mutants for all dispensable genes
of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were …

Genome plasticity in Acinetobacter: new degradative capabilities acquired by the spontaneous amplification of large chromosomal segments

AB Reams, EL Neidle - Molecular Microbiology, 2003 - Wiley Online Library
In Acinetobacter sp. ADP1, growth on benzoate requires regulation of the cat genes by two
transcriptional activators. Here, mutants were obtained from a strain lacking both activators …