Standard freezing protocols of clinically relevant cell lines commonly employ agents such as
fetal bovine serum and dimethyl sulfoxide, which are a potential concern from both a …

Human mesenchymal stem cells (hMSCs) have some favorable characteristics like high
plasticity, multilineage differentiation potential, and comparably easy handling in vitro …

Abstract Background and Aim An essential aspect of ensuring availability and stability of
mesenchymal stem/stromal cells (MSCs) products for clinical use is that these cells are …

The aim of this study is to investigate the feasibility of using well defined, serum‐free
freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5%(v/v) …

Experimental and clinical applications of mesenchymal stromal cells (MSCs) require the
development of new approaches and improvements of existing methods of cell …

The advent of stem cells and stem cell-based therapies for specific diseases requires
particular knowledge of laboratory procedures, which not only guarantee the continuous …

Abstract Purpose of Review Cryopreservation and its associated freezing and thawing
procedures–short “freeze-thawing”–are among the final steps in economically viable …

Mesenchymal stem cells (MSCs) are able to differentiate in vivo and in vitro giving rise to
different cell types including osteoblasts, adipocytes, chondrocytes and neuronal cells …

With the wide-spread possibility of clinical applications of cell-based therapies, there is an
obvious need to develop long-term storage strategies for these cell-based products. At …

Over the past few years, the pace of preclinical stem cell research is astonishing and adult
stem cells have become the subject of intense research. Due to the presence of promising …