[HTML][HTML] Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

E Martínez-García, PI Nikel, T Aparicio… - Microbial cell …, 2014 - Springer
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
By the time the complete genome sequence of the soil bacterium Pseudomonas putida
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …

[HTML][HTML] Genome reduction boosts heterologous gene expression in Pseudomonas putida

S Lieder, PI Nikel, V de Lorenzo, R Takors - Microbial cell factories, 2015 - Springer
Background The implementation of novel platform organisms to be used as microbial cell
factories in industrial applications is currently the subject of intense research. Ongoing …

Identification of conditionally essential genes for growth of Pseudomonas putida KT2440 on minimal medium through the screening of a genome‐wide mutant library

MA Molina‐Henares, J De La Torre… - Environmental …, 2010 - Wiley Online Library
In silico models for Pseudomonas putida KT2440 metabolism predict 68 genes to be
essential for growth on minimal medium. In this study a genome‐wide collection of single …

[HTML][HTML] Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform

E Martínez-García, V de Lorenzo - Current opinion in biotechnology, 2024 - Elsevier
Highlights•Pseudomonas putida has consolidated as areliable Synthetic Biology
chassis.•Genome-reduction often enhances performance of P. putida strains.• …

[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

KE Nelson, C Weinel, IT Paulsen… - Environmental …, 2002 - Wiley Online Library
Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been
certified as a biosafety host for the cloning of foreign genes. The bacterium also has …

Pseudomonas putida KT2440 is naturally endowed to withstand industrial‐scale stress conditions

A Ankenbauer, RA Schäfer, SC Viegas… - Microbial …, 2020 - Wiley Online Library
Pseudomonas putida is recognized as a very promising strain for industrial application due
to its high redox capacity and frequently observed tolerance towards organic solvents. In this …

Tn7-Based Device for Calibrated Heterologous Gene Expression in Pseudomonas putida

S Zobel, I Benedetti, L Eisenbach… - ACS synthetic …, 2015 - ACS Publications
The soil bacterium Pseudomonas putida is increasingly attracting considerable interest as a
platform for advanced metabolic engineering through synthetic biology approaches …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …