[HTML][HTML] Whole-cell bioreduction of aromatic α-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in …
R Kratzer, M Pukl, S Egger, B Nidetzky - Microbial Cell Factories, 2008 - Springer
Background Whole cell-catalyzed biotransformation is a clear process option for the
production of chiral alcohols via enantioselective reduction of precursor ketones. A wide …
production of chiral alcohols via enantioselective reduction of precursor ketones. A wide …
Identification of Candida tenuis xylose reductase as highly selective biocatalyst for the synthesis of aromatic α-hydroxy esters and improvement of its efficiency by …
R Kratzer, B Nidetzky - Chemical communications, 2007 - pubs.rsc.org
Wild-type Candida tenuisxylose reductase and two Trp-23 mutants thereof catalyze NADH-
dependent reduction of a homologous series of aromatic α-keto esters with absolute pseudo …
dependent reduction of a homologous series of aromatic α-keto esters with absolute pseudo …
Enzyme identification and development of a whole‐cell biotransformation for asymmetric reduction of o‐chloroacetophenone
R Kratzer, M Pukl, S Egger, M Vogl… - Biotechnology and …, 2011 - Wiley Online Library
Abstract Chiral 1‐(o‐chlorophenyl)‐ethanols are key intermediates in the synthesis of
chemotherapeutic substances. Enantioselective reduction of o‐chloroacetophenone is a …
chemotherapeutic substances. Enantioselective reduction of o‐chloroacetophenone is a …
Biocatalytic ketone reduction—a powerful tool for the production of chiral alcohols—part II: whole-cell reductions
Enzymes are able to perform reactions under mild conditions, eg, pH and temperature, with
remarkable chemo-, regio-, and stereoselectivity. Due to this feature the number of …
remarkable chemo-, regio-, and stereoselectivity. Due to this feature the number of …
Strain engineering for stereoselective bioreduction of dicarbonyl compounds by yeast reductases
T Johanson, M Katz… - FEMS yeast …, 2005 - academic.oup.com
Pure chiral molecules are needed in the pharmaceutical and chemical industry as
intermediates for the production of drugs or fine chemicals. Microorganisms represent an …
intermediates for the production of drugs or fine chemicals. Microorganisms represent an …
Integration of enzyme, strain and reaction engineering to overcome limitations of baker's yeast in the asymmetric reduction of α‐keto esters
R Kratzer, S Egger, B Nidetzky - Biotechnology and …, 2008 - Wiley Online Library
We report on the development of a whole‐cell biocatalytic system based on the popular host
Saccharomyces cerevisiae that shows programmable performance and good atom economy …
Saccharomyces cerevisiae that shows programmable performance and good atom economy …
Asymmetric reduction of α, β‐unsaturated ketone to (R) allylic alcohol by Candida chilensis
DJ Pollard, K Telari, J Lane… - Biotechnology and …, 2006 - Wiley Online Library
A pilot scale whole cell process was developed for the enantioselective 1, 2‐reduction of
prochiral α, β‐unsaturated ketone to (R) allylic alcohol using Candida chilensis. Initial …
prochiral α, β‐unsaturated ketone to (R) allylic alcohol using Candida chilensis. Initial …
[HTML][HTML] Whole-cell biotransformation systems for reduction of prochiral carbonyl compounds to chiral alcohol in Escherichia coli
B Li, Y Li, D Bai, X Zhang, H Yang, J Wang, G Liu… - Scientific reports, 2014 - nature.com
Lactobacillus brevis alcohol dehydrogenase (Lb-ADH) catalyzes reduction of prochiral
carbonyl compounds to chiral alcohol and meanwhile consumes its cofactor NADH into …
carbonyl compounds to chiral alcohol and meanwhile consumes its cofactor NADH into …
Genome hunting of carbonyl reductases from Candida glabrata for efficient preparation of chiral secondary alcohols
G Xu, Y Zhang, Y Wang, Y Ni - Bioresource technology, 2018 - Elsevier
In this work, genome hunting strategy was adopted in screening for reductases from
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli …
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli …
New strategy for effective biosynthesis of chiral aryl alcohols: Co-cultivation microbe with natural deep-eutectic solvent
F Qian, HY Liu, SR Yu, Y Zhang… - … Sustainable Chemistry & …, 2023 - ACS Publications
In this study, a new strategy was developed for efficient synthesis of chiral aryl alcohols
mediated by permeabilizing whole-cell catalyzed bioreduction. The permeabilized …
mediated by permeabilizing whole-cell catalyzed bioreduction. The permeabilized …