Single-cell transcriptomics provide a systematic map of gene expression in different human
cell types. The next challenge is to systematically understand cell-type-specific gene …

CRISPR/Cas9-based functional genomics have transformed our ability to elucidate
mammalian cell biology. However, most previous CRISPR-based screens were conducted …

Microglia are emerging as key drivers of neurological diseases. However, we lack a
systematic understanding of the underlying mechanisms. Here, we present a screening …

The ability to reprogram adult somatic cells into induced pluripotent stem cells (iPSCs) and
the subsequent development of protocols for their differentiation into disease-relevant cell …

The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for
genome editing in eukaryotic cells. This technique allows for sequence-specific induction of …

Neurodegenerative, neurodevelopmental and neuropsychiatric disorders are among the
greatest public health challenges, as many lack disease-modifying treatments. A major …

CRISPR activation (CRISPRa) is an important tool to perturb transcription, but its
effectiveness varies between target genes. We employ human pluripotent stem cells with …

Next-generation DNA sequencing technologies have led to a massive accumulation of
genomic and transcriptomic data from patients and healthy individuals. The major challenge …

CRISPR (clustered regularly interspaced short palindromic repeats)-based gene inactivation
provides a powerful means for linking genes to particular cellular phenotypes. CRISPR …

Genome-wide CRISPR screens have transformed our ability to systematically interrogate
human gene function, but are currently limited to a subset of cellular phenotypes. We report …