Development of an aggregation-based immunoassay for anti-protein A using gold nanoparticles

NTK Thanh, Z Rosenzweig - Analytical chemistry, 2002 - ACS Publications
A unique, sensitive, and highly specific immunoassay system for antibodies using gold
nanoparticles has been developed. The assay is based on the aggregation of gold …

Characterization and optimization of gold nanoparticle-based silver-enhanced immunoassays

S Gupta, S Huda, PK Kilpatrick, OD Velev - Analytical Chemistry, 2007 - ACS Publications
Silver-enhanced nanoparticle-labeled immunoassays provide a simple, low-cost, and
effective way of detecting antigens in dilute solutions. The physical mechanisms behind their …

A protein A-based orientation-controlled immobilization strategy for antibodies using nanometer-sized gold particles and plasma-polymerized film

H Wang, Y Liu, Y Yang, T Deng, G Shen, R Yu - Analytical Biochemistry, 2004 - Elsevier
A novel protein A (PA)-based strategy for the orientation-controlled immobilization of
antibodies using nanometer-sized gold (Nano-gold) particles and an amine-terminated …

A versatile SERS-based immunoassay for immunoglobulin detection using antigen-coated gold nanoparticles and malachite green-conjugated protein A/G

J Neng, MH Harpster, H Zhang, JO Mecham… - Biosensors and …, 2010 - Elsevier
A surface enhanced Raman scattering (SERS) immunoassay for antibody detection in
serum is described in the present work. The developed assay is conducted in solution and …

Single chain fragment variable recombinant antibody functionalized gold nanoparticles for a highly sensitive colorimetric immunoassay

Y Liu, Y Liu, RL Mernaugh, X Zeng - Biosensors and Bioelectronics, 2009 - Elsevier
In this report, the peptide linker connecting scFv VH and VL domains were genetically
modified to contain different amino acids (ie cysteine (scFv-cys) or histidines (scFv-his)) to …

Gold-coated magnetic particles for solid-phase immunoassays: enhancing immobilized antibody binding efficiency and analytical performance

H Zhang, ME Meyerhoff - Analytical chemistry, 2006 - ACS Publications
The preparation and characterization of gold-coated magnetic particles are described for
use as more efficient solid-phase materials in immunoassay development. A thin gold …

A washing-free and amplification-free one-step homogeneous assay for protein detection using gold nanoparticle probes and dynamic light scattering

X Liu, Q Huo - Journal of immunological methods, 2009 - Elsevier
In this study, we developed a one-step, washing-free and amplification-free assay for protein
analysis using gold nanoparticle probes (GNPs) and dynamic light scattering (DLS) …

Gold nanoparticle-based immunochromatographic test for identification of Staphylococcus aureus from clinical specimens

SH Huang - Clinica chimica acta, 2006 - Elsevier
BACKGROUND: Staphylococcus aureus is one of the most important human pathogens,
causing both nosocomial and community-acquired infections. Therefore, a method for …

A new protein A assay based on Raman reporter labeled immunogold nanoparticles

CC Lin, YM Yang, YF Chen, TS Yang… - Biosensors and …, 2008 - Elsevier
A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system
utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described …

Bioconjugation and characterisation of gold colloid-labelled proteins

S Thobhani, S Attree, R Boyd, N Kumarswami… - Journal of …, 2010 - Elsevier
Colloidal metal particles, in particular gold, have found many biological applications often as
probes in light and electron microscopy, and more recently since the 1980s in membrane …