$1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen‐rich samples

R Meier, W Wong, A Srivathsan, M Foo - Cladistics, 2016 - Wiley Online Library
Several of the biggest challenges in taxonomy and systematics are related to a toxic mixture
of small size, abundance, and rarity. There are too many species in groups with too few …

'Direct PCR'optimization yields a rapid, cost‐effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction

WH Wong, YC Tay, J Puniamoorthy… - Molecular Ecology …, 2014 - Wiley Online Library
Macroinvertebrates that are collected in large numbers pose major problems in basic and
applied biodiversity research: identification to species via morphology is often difficult, slow …

A comprehensive DNA sequence library is essential for identification with DNA barcodes

T Ekrem, E Willassen, E Stur - Molecular phylogenetics and evolution, 2007 - Elsevier
In this study we examine the possibility of utilising partial cox1 gene sequences as barcodes
to identify non-biting midges (Diptera: Chironomidae). We analysed DNA from 97 specimens …

Exploring genetic divergence in a species-rich insect genus using 2790 DNA barcodes

X Lin, E Stur, T Ekrem - PloS one, 2015 - journals.plos.org
DNA barcoding using a fragment of the mitochondrial cytochrome c oxidase subunit 1 gene
(COI) has proven to be successful for species-level identification in many animal groups …

DNA barcodes and morphology reveal unrecognized species in Chironomidae (Diptera)

XL Lin, E Stur, T Ekrem - Insect Systematics & Evolution, 2018 - brill.com
For over a decade, DNA barcoding has proven an effective modern tool in taxonomy,
evolutionary biology and biodiversity research. Many new species have been discovered …

Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

A Srivathsan, E Hartop, J Puniamoorthy, WT Lee… - BMC biology, 2019 - Springer
Background More than 80% of all animal species remain unknown to science. Most of these
species live in the tropics and belong to animal taxa that combine small body size with high …

DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success

R Meier, K Shiyang, G Vaidya, PKL Ng - Systematic biology, 2006 - academic.oup.com
DNA barcoding and DNA taxonomy have recently been proposed as solutions to the crisis of
taxonomy and received significant attention from scientific journals, grant agencies, natural …

DNA barcodes successfully delimit morphospecies in a superdiverse insect genus

C Song, XL Lin, Q Wang, XH Wang - Zoologica Scripta, 2018 - Wiley Online Library
Polypedilum Kieffer (Diptera: Chironomidae), with 520 currently known species worldwide,
can be extremely difficult to identify species level based on the morphology. We used 3,670 …

One species in eight: DNA barcodes from type specimens resolve a taxonomic quagmire

M Mutanen, M Kekkonen, SWJ Prosser… - Molecular Ecology …, 2015 - Wiley Online Library
Each holotype specimen provides the only objective link to a particular Linnean binomen.
Sequence information from them is increasingly valuable due to the growing usage of DNA …

Longer is not always better: Optimizing barcode length for large-scale species discovery and identification

D Yeo, A Srivathsan, R Meier - Systematic Biology, 2020 - academic.oup.com
New techniques for the species-level sorting of millions of specimens are needed in order to
accelerate species discovery, determine how many species live on earth, and develop …