Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Background It is widely believed that laboratory strains of Escherichia coli, including those
used for industrial production of proteins, do not secrete proteins to the extracellular milieu …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Background The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative
bacteria, combines a relatively simple protein secretion mechanism with a high transport …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

Autotransporters constitute a large family of natural proteins that are essential for delivering
many types of proteins and peptides across the outer membrane in Gram-negative bacteria …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …

Bacterial autotransporters are proteins that contain a small C‐terminal 'β domain'that
facilitates translocation of a large N‐terminal 'passenger domain'across the outer membrane …