Highlights•Combining enzyme engineering and pathway design allows for many methanol
assimilation routes.•Several, reduced products can be synthesized from a mixture of sugar …

Abstract One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing
industry and have recently gained attention owing to their natural abundance, low …

Abstract One-carbon (C1) feedstocks can provide a vital link between cheap and
sustainable abiotic resources and microbial bioproduction. Soluble C1 substrates, methanol …

Carbon dioxide (CO2) stands out as sustainable feedstock for developing a circular carbon
economy whose energy supply could be obtained by boosting the production of clean …

Microbial fermentation processes are expected to play an important role in reducing
dependence on fossil-based raw materials for the production of everyday chemicals. In …

The use of abundant and cheap one carbon (C1) feedstocks to produce value-added
chemicals is an important approach for achieving carbon neutrality and tackling …

Highlights•One-carbon compounds can be made from CO 2 to provide renewable microbial
feedstocks.•Methanol and formate have key advantages over gaseous one-carbon …

Engineering microorganisms to grow on alternative feedstocks is crucial not just because of
the indisputable biotechnological applications but also to deepen our understanding of …

Highlights•Synthetic methylotrophy achieved in E. coli via ribulose monophosphate
cycle.•Synthetic methylotrophy has been achieved in E. coli via reductive glycine …

An increasing focus on environmental sustainability has led to an ongoing global effort to
better understand and engineer platform organisms that economically convert CO 2 and …