Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
The ability to artificially control transcription is essential both to the study of gene function
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …
CRISPR tools to control gene expression in bacteria
A Vigouroux, D Bikard - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
SUMMARY CRISPR-Cas systems have been engineered as powerful tools to control gene
expression in bacteria. The most common strategy relies on the use of Cas effectors …
expression in bacteria. The most common strategy relies on the use of Cas effectors …
[HTML][HTML] Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria
C Dong, J Fontana, A Patel, JM Carothers… - Nature …, 2018 - nature.com
Methods to regulate gene expression programs in bacterial cells are limited by the absence
of effective gene activators. To address this challenge, we have developed synthetic …
of effective gene activators. To address this challenge, we have developed synthetic …
Programmable CRISPR‐Cas transcriptional activation in bacteria
HI Ho, JR Fang, J Cheung, HH Wang - Molecular systems biology, 2020 - embopress.org
Programmable gene activation enables fine‐tuned regulation of endogenous and synthetic
gene circuits to control cellular behavior. While CRISPR‐Cas‐mediated gene activation has …
gene circuits to control cellular behavior. While CRISPR‐Cas‐mediated gene activation has …
CRISPR-Cas activators for engineering gene expression in higher eukaryotes
JA Casas-Mollano, MH Zinselmeier… - The CRISPR …, 2020 - liebertpub.com
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce
expression of one or many target genes in trans. Here we review the many design variations …
expression of one or many target genes in trans. Here we review the many design variations …
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
ML Luo, AS Mullis, RT Leenay… - Nucleic acids research, 2015 - academic.oup.com
CRISPR-Cas systems have shown tremendous promise as heterologous tools for genome
editing and transcriptional regulation. Because these RNA-directed immune systems are …
editing and transcriptional regulation. Because these RNA-directed immune systems are …
RNA-guided gene activation by CRISPR-Cas9–based transcription factors
Technologies for engineering synthetic transcription factors have enabled many advances in
medical and scientific research. In contrast to existing methods based on engineering of …
medical and scientific research. In contrast to existing methods based on engineering of …
[HTML][HTML] Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
Transcriptional regulation by nuclease-deficient CRISPR/Cas is a popular and valuable tool
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells
Class 2 CRISPR–Cas systems, such as Cas9 and Cas12, have been widely used to target
DNA sequences in eukaryotic genomes. However, class 1 CRISPR–Cas systems, which …
DNA sequences in eukaryotic genomes. However, class 1 CRISPR–Cas systems, which …
Cas9-based tools for targeted genome editing and transcriptional control
Development of tools for targeted genome editing and regulation of gene expression has
significantly expanded our ability to elucidate the mechanisms of interesting biological …
significantly expanded our ability to elucidate the mechanisms of interesting biological …