Diazirines are among the smallest photoreactive groups that form a reactive carbene upon
light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand …

Diazirines are three-membered ring systems containing two nitrogen atoms and one carbon
atom1, 2 widely applicable in biology as photoaffinity labeling agents. 3, 4 Diazirines can be …

Target identification is a high-priority, albeit challenging, aspect of drug discovery. Diazirine-
based photoaffinity probes (PAPs) can facilitate the process by covalently capturing transient …

A series of simple methods formodifying diazirines bearing an aromatic ring has
beenaccomplished. This first versatile approach involving direct substitution on the aromatic …

The elucidation of substrate–protein interactions is an important component of the drug
development process. Due to the complexity of native cellular environments, elucidating …

As a result of recent developments in molecular biology, the investigation of biofunctional
machinery at ligand‐accepting interfaces has become one of the challenging and important …

Diazirines are widely used in photoaffinity labeling (PAL) to trap noncovalent interactions
with biomolecules. However, design and interpretation of PAL experiments is challenging …

Alkyl diazirines are frequently used in photoaffinity labeling to map small molecule–protein
interactions in target identification studies. However, the alkyl diazirines can preferentially …

Target identification is often the rate‐determining step in deciphering the mechanism of
action of biologically active small molecules. Photo‐affinity labeling (PAL) represents a …

The use of photo-affinity reagents for the mapping of noncovalent small molecule–protein
interactions has become widespread. Recently, several 'fully-functionalized'(FF) chemical …