Detailed Structural Characterization of Glucans Produced by Glucansucrases from Leuconostoc citreum TMW 2.1194

F Münkel, J Bechtner, V Eckel, A Fischer… - Journal of agricultural …, 2019 - ACS Publications
The water kefir organism Leuconostoc citreum TMW 2.1194 forms highly branched dextrans
with O 3-and O 4-bound side chains. To obtain detailed information on the enzymatic …

Cloning, expression, and characterization of an insoluble glucan-producing glucansucrase from Leuconostoc mesenteroides NRRL B-1118

GL Côté, CD Skory - Applied microbiology and biotechnology, 2012 - Springer
We have cloned a glucansucrase from the type strain of Leuconostoc mesenteroides (NRRL
B-1118; ATCC 8293) and successfully expressed the enzyme in Escherichia coli. The …

Isolation and partial characterization of an extracellular glucansucrase from Leuconostoc mesenteroides NRRL B-1355 that synthesizes an alternating (1→ 6),(1→ 3) …

GL CôTÉ, JF Robyt - Carbohydrate research, 1982 - Elsevier
Leuconostoc mesenteroides NRRL B-1355 grows on sucrose to produce two extracellular α-
d-glucans. Although both are termed dextrans, they are chemically and physically distinct …

Characterisation of insoluble α-1, 3-/α-1, 6 mixed linkage glucan produced in addition to soluble α-1, 6-linked dextran by glucansucrase (DEX-N) from Leuconostoc …

K Wangpaiboon, N Waiyaseesang, P Panpetch… - International journal of …, 2020 - Elsevier
Glucansucrases catalyse the formation of glucans from sucrose. The glucansucrase-
encoding gene from Leuconostoc citreum ABK-1, dex-N, was successfully cloned and …

Structural characterization of mixed-linkage α-glucans produced by mutants of Lactobacillus reuteri TMW 1.106 dextransucrase

F Münkel, A Fischer, D Wefers - Carbohydrate polymers, 2020 - Elsevier
Dextrans and other bacterial α-glucans are versatile and structurally diverse
polysaccharides which can be enzymatically synthesized by using glucansucrases. By …

Synthesis of methyl α-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299

AG De Segura, M Alcalde, M Bernabé… - Journal of …, 2006 - Elsevier
The synthesis of methyl α-d-glucooligosaccharides, using sucrose as glucosyl donor and
methyl α-d-glucopyranoside as acceptor, was studied with dextransucrase from …

Water-soluble and water-insoluble glucans produced by Escherichia coli recombinant dextransucrases from Leuconostoc mesenteroides NRRL B-512F

K Funane, T Ishii, M Matsushita, K Hori, K Mizuno… - Carbohydrate …, 2001 - Elsevier
Two dextransucrase genes, dsrS and dsrT5, from Leuconostoc mesenteroides NRRL B-
512F were expressed in Escherichia coli, and recombinant dsrT5 dextransucrase was …

[HTML][HTML] Changes in linkage pattern of glucan products induced by substitution of Lys residues in the dextransucrase

K Funane, T Ishii, H Ono, M Kobayashi - FEBS letters, 2005 - Elsevier
Dextransucrase S (DSRS) is the only active glucansucrase that has been found in
Leuconostoc mesenteroides NRRL B-512F strain. Native DSRS produces mainly 6-linked …

Analysis of Structural and Functional Differences of Glucans Produced by the Natively Released Dextransucrase of Liquorilactobacillus hordei TMW 1.1822

J Schmid, D Wefers, RF Vogel, F Jakob - Applied biochemistry and …, 2021 - Springer
The properties of the glucopolymer dextran are versatile and linked to its molecular size,
structure, branching, and secondary structure. However, suited strategies to control and …

Leuconostoc citreum SK24. 002 glucansucrase: Biochemical characterisation and de novo synthesis of α-glucan

L Song, M Miao, B Jiang, T Xu, SW Cui… - International journal of …, 2016 - Elsevier
The cell-associated glucansucrase from Leuconostoc citreum SK24. 002 was isolated,
purified, characterized and used for de novo synthesis of α-glucan and acceptor-products …