The top-down approach to proteomics offers compelling advantages due to the potential to
provide complete characterization of protein sequence and post-translational modifications …

The analysis of intact proteins (top-down strategy) by mass spectrometry has great potential
to elucidate proteoform variation, including patterns of post-translational modifications …

Proteomics applications performed on the popular benchtop Q Exactive Orbitrap mass
spectrometer have so far relied exclusively on higher collision-energy dissociation (HCD) …

The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has
ushered in a new age of promise and challenge for the characterization and identification of …

Although only a few years old, the combination of a linear ion trap with an Orbitrap analyzer
has become one of the standard mass spectrometers to characterize proteins and …

Ultraviolet photodissociation (UVPD) of gas-phase proteins has attracted increased attention
in recent years. This growing interest is largely based on the fact that, in contrast to slow …

Top-down proteomics studies intact proteoform mixtures and offers important advantages
over more common bottom-up proteomics technologies, as it avoids the protein inference …

The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Mass spectrometry-based proteomics has greatly benefitted from enormous advances in
high resolution instrumentation in recent years. In particular, the combination of a linear ion …

Electron-based dissociation (ExD) produces uncluttered mass spectra of intact proteins
while preserving labile post-translational modifications. However, technical challenges have …