The ability of Escherichia coli and Bacillus subtilis to regulate their cytoplasmic pH is well
studied in cell suspensions but is poorly understood in individual adherent cells and …

The development of a rapid method for measuring intracellular pH (pHi) in single bacterial
cells is described. Lactobacillus delbrueckii subsp. bulgaricus and Listeria innocua were …

We show that a pH-sensitive derivative of the green fluorescent protein, designated
ratiometric GFP, can be used to measure intracellular pH (pHi) in both gram-positive and …

Cytoplasmic pH and periplasmic pH of Escherichia coli cells in suspension were observed
with 4-s time resolution using fluorimetry of TorA-green fluorescent protein mutant 3*(TorA …

Biofilms are comprised of bacterial-clusters (microcolonies) enmeshed in an extracellular
matrix. Streptococcus mutans can produce exopolysaccharides (EPS)-matrix and assemble …

We describe the dynamics of changes in the intracellular pH (pHi) values of a number of
lactic acid bacteria in response to a rapid drop in the extracellular pH (pHex). Strains of …

Confocal microscopy-based monitoring of pH in biofilms is gaining increasing interest, as it
allows for a quick assessment of horizontal pH gradients without mechanically perturbing …

An important feature of microbial biofilms is the development of four-dimensional physical
and chemical gradients in space and time. There is need for novel approaches to probe …

In this study we investigated the relationship between a rapid change in extracellular pH and
the alteration of bacterial ATP concentration. This relationship is a key component of a …

The ability of cells to maintain pH homeostasis in response to environmental changes has
elicited interest in basic and applied research and has prompted the development of …