Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low fertilization ability in Ban—A native Vietnamese pigs

NV Linh, T Somfai, TH Nguyen, NT Nhung… - Animal Science …, 2018 - Wiley Online Library
The aim of the present study was to improve the penetration during in vitro fertilization (IVF)
of a frozen lot of epididymal sperm with a notoriously low fertilization ability of a Ban boar …

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs

Z Namula, Y Isumi, Y Sato, QA Le, Q Lin… - Archives Animal …, 2021 - aab.copernicus.org
This study aimed to compare the quality and the penetration ability of frozen–thawed
spermatozoa from three microminipigs and Large White boars and to evaluate the effects of …

Artificial insemination with seminal plasma improves the reproductive performance of frozen‐thawed boar epididymal spermatozoa

T Okazaki, T Akiyoshi, M Kan, M Mori… - Journal of …, 2012 - Wiley Online Library
Frozen‐thawed epididymal spermatozoa have good fertilization capability in vitro; however,
their artificial insemination conception rate is less than half of that of frozen‐thawed …

High pre-freezing sperm dilution improves monospermy without affecting the penetration rate in porcine IVF

CA Martinez, JM Cambra, C Maside, C Cuello, J Roca… - Theriogenology, 2019 - Elsevier
The high incidence of polyspermy is still an unresolved problem for the production of in vitro-
produced porcine embryos. In this work, we modified the usual sperm processing sequence …

Liquid boar sperm quality during storage and in vitro fertilization and culture of pig oocytes

CS Park, MY Kim, YJ Yi, YJ Chang, SH Lee… - … journal of animal …, 2004 - koreascience.kr
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted
and preserved at $4^{\circ} C $ with lactose hydrate, egg yolk and N-acetyl-D-glucosamine …

Effect of preincubation of cryopreserved porcine epididymal sperm

H Ikeda, K Kikuchi, J Noguchi, H Takeda, A Shimada… - Theriogenology, 2002 - Elsevier
Preincubation of spermatozoa is important for capacitation and successful fertilization in
vitro. The effects of preincubation time on frozen-thawed boar epididymal spermatozoa as …

Methods for Improving In Vitro and In Vivo Boar Sperm Fertility

H Funahashi - Reproduction in Domestic Animals, 2015 - Wiley Online Library
Contents Fertility of boar spermatozoa is changed after ejaculation in vivo and in vitro.
During processing for in vitro fertilization (IVF), although spermatozoa are induced …

In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws

YJ Yi, HJ Ko, SH Lee, CB Yang, DS Son… - … -australasian journal of …, 2004 - animbiosci.org
This study was carried out to investigate in vitro fertilization and development of in vitro
matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing …

Adjustments in IVF system for individual boars: value of additives and time of sperm–oocyte co-incubation

C Almiñana, MA Gil, C Cuello, J Roca, JM Vazquez… - Theriogenology, 2005 - Elsevier
In vitro fertilization (IVF) in pigs is still considered sub-optimal, due to the variable
occurrence of polyspermy, variability mainly related to sperm differences. The present study …

In vitro fertilization of porcine oocytes with fresh and frozen-thawed ejaculated or frozen-thawed epididymal semen obtained from identical boars

D Rath, H Niemann - Theriogenology, 1997 - Elsevier
The objective of this study was to compare the in vitro fertilizing capacity of porcine
spermatozoa from fresh and frozen-thawed semen and frozen-thawed epididymal …