[HTML][HTML] Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc
S Watanabe, T Kodaki, K Makino - Journal of Biological Chemistry, 2005 - ASBMB
Pichia stipitisNAD+-dependent xylitol dehydrogenase (XDH), a medium-chain
dehydrogenase/reductase, is one of the key enzymes in ethanol fermentation from xylose …
dehydrogenase/reductase, is one of the key enzymes in ethanol fermentation from xylose …
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase
Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol
dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were …
dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were …
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein-engineered NADH-preferring xylose reductase from Pichia stipitis
A recombinant Saccharomyces cerevisiae strain transformed with xylose reductase (XR)
and xylitol dehydrogenase (XDH) genes from Pichia stipitis (PsXR and PsXDH, respectively) …
and xylitol dehydrogenase (XDH) genes from Pichia stipitis (PsXR and PsXDH, respectively) …
Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme‐Binding Domain Affect the Coenzyme Specificity
MH Metzger, CP Hollenberg - European journal of biochemistry, 1995 - Wiley Online Library
Directed mutagenesis has been used to identify a set of amino acids in the Pichia stipitis
xylitol dehydrogenase, encoded by the xylitol dehydrogenase gene XYL2, which is involved …
xylitol dehydrogenase, encoded by the xylitol dehydrogenase gene XYL2, which is involved …
[HTML][HTML] Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity
AH Ehrensberger, RA Elling, DK Wilson - Structure, 2006 - cell.com
Xylitol dehydrogenase (XDH) is one of several enzymes responsible for assimilating xylose
into eukaryotic metabolism and is useful for fermentation of xylose contained in agricultural …
into eukaryotic metabolism and is useful for fermentation of xylose contained in agricultural …
Reversal of coenzyme specificity and improvement of catalytic efficiency of Pichia stipitis xylose reductase by rational site-directed mutagenesis
A major problem when xylose is used for ethanol production is the intercellular redox
imbalance arising from different coenzyme specificities of xylose reductase (XR) and xylitol …
imbalance arising from different coenzyme specificities of xylose reductase (XR) and xylitol …
[HTML][HTML] Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
O Bengtsson, B Hahn-Hägerdal… - Biotechnology for …, 2009 - Springer
Background Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are
the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains …
the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains …
Expression of protein engineered NADP+-dependent xylitol dehydrogenase increases ethanol production from xylose in recombinant Saccharomyces cerevisiae
A Matsushika, S Watanabe, T Kodaki, K Makino… - Applied microbiology …, 2008 - Springer
A recombinant Saccharomyces cerevisiae strain transformed with xylose reductase (XR)
and xylitol dehydrogenase (XDH) genes from Pichia stipitis has the ability to convert xylose …
and xylitol dehydrogenase (XDH) genes from Pichia stipitis has the ability to convert xylose …
The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant …
We focused on the effects of a mutation of xylose reductase from Pichia stipitis (Ps XR) on
xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed …
xylose-to-ethanol fermentation using recombinant Saccharomyces cerevisiae transformed …
[HTML][HTML] Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of …
B Petschacher, B Nidetzky - Microbial cell factories, 2008 - Springer
Abstract Background Metabolic engineering of Saccharomyces cerevisiae for xylose
fermentation into fuel ethanol has oftentimes relied on insertion of a heterologous pathway …
fermentation into fuel ethanol has oftentimes relied on insertion of a heterologous pathway …
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