Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein
R Yoshida, S Ozaki, H Kawakami… - Nucleic acids …, 2023 - academic.oup.com
The Escherichia coli replication origin oriC contains the initiator ATP-DnaA-Oligomerization
Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion …
Region (DOR) and its flanking duplex unwinding element (DUE). In the Left-DOR subregion …
Regulatory dynamics in the ternary DnaA complex for initiation of chromosomal replication in Escherichia coli
Y Sakiyama, K Kasho, Y Noguchi… - Nucleic acids …, 2017 - academic.oup.com
In Escherichia coli, the level of the ATP–DnaA initiator is increased temporarily at the time of
replication initiation. The replication origin, oriC, contains a duplex-unwinding element …
replication initiation. The replication origin, oriC, contains a duplex-unwinding element …
Highly organized DnaA– oriC complexes recruit the single-stranded DNA for replication initiation
S Ozaki, T Katayama - Nucleic acids research, 2012 - academic.oup.com
In Escherichia coli, the replication origin oriC consists of two functional regions: the duplex
unwinding element (DUE) and its flanking DnaA-assembly region (DAR). ATP-DnaA …
unwinding element (DUE) and its flanking DnaA-assembly region (DAR). ATP-DnaA …
[HTML][HTML] The Arg fingers of key DnaA protomers are oriented inward within the replication origin oriC and stimulate DnaA subcomplexes in the initiation complex
Y Noguchi, Y Sakiyama, H Kawakami… - Journal of Biological …, 2015 - ASBMB
ATP-DnaA binds to multiple DnaA boxes in the Escherichia coli replication origin (oriC) and
forms left-half and right-half subcomplexes that promote DNA unwinding and DnaB helicase …
forms left-half and right-half subcomplexes that promote DNA unwinding and DnaB helicase …
[HTML][HTML] Differentiation of the DnaA-oriC subcomplex for DNA unwinding in a replication initiation complex
S Ozaki, Y Noguchi, Y Hayashi, E Miyazaki… - Journal of Biological …, 2012 - ASBMB
In Escherichia coli, ATP-DnaA multimers formed on the replication origin oriC promote
duplex unwinding, which leads to helicase loading. Based on a detailed functional analysis …
duplex unwinding, which leads to helicase loading. Based on a detailed functional analysis …
[HTML][HTML] The DnaA Cycle in Escherichia coli: Activation, Function and Inactivation of the Initiator Protein
T Katayama, K Kasho, H Kawakami - Frontiers in microbiology, 2017 - frontiersin.org
This review summarizes the mechanisms of the initiator protein DnaA in replication initiation
and its regulation in Escherichia coli. The chromosomal origin (oriC) DNA is unwound by the …
and its regulation in Escherichia coli. The chromosomal origin (oriC) DNA is unwound by the …
[HTML][HTML] Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading
Y Sakiyama, M Nagata, R Yoshida, K Kasho… - Journal of Biological …, 2022 - ASBMB
Unwinding of the replication origin and loading of DNA helicases underlie the initiation of
chromosomal replication. In Escherichia coli, the minimal origin oriC contains a duplex …
chromosomal replication. In Escherichia coli, the minimal origin oriC contains a duplex …
[HTML][HTML] The DnaA AAA+ Domain His136 Residue Directs DnaB Replicative Helicase to the Unwound Region of the Replication Origin, oriC
Y Sakiyama, M Nishimura, C Hayashi… - Frontiers in …, 2018 - frontiersin.org
Chromosomal replication initiation requires dynamic mechanisms in higher-order
nucleoprotein complexes that are constructed at the origin of replication. In Escherichia coli …
nucleoprotein complexes that are constructed at the origin of replication. In Escherichia coli …
[HTML][HTML] An essential tryptophan of Escherichia coli DnaA protein functions in oligomerization at the E. coli replication origin
MM Felczak, LA Simmons, JM Kaguni - Journal of Biological Chemistry, 2005 - ASBMB
In the initiation of bacterial DNA replication, DnaA protein recruits DnaB helicase to the
chromosomal origin, oriC, leading to the assemble of the replication fork machinery at this …
chromosomal origin, oriC, leading to the assemble of the replication fork machinery at this …
Structural basis of replication origin recognition by the DnaA protein
N Fujikawa, H Kurumizaka, O Nureki… - Nucleic acids …, 2003 - academic.oup.com
Escherichia coli DnaA binds to 9 bp sequences (DnaA boxes) in the replication origin, oriC,
to form a complex initiating chromosomal DNA replication. In the present study, we …
to form a complex initiating chromosomal DNA replication. In the present study, we …