There is a clear need for developments in characterisation techniques that provide detailed
information about structure–function relationships in biology. Using electron microscopy to …

Cryo-transmission electron microscopy (cryo-EM) of frozen hydrated specimens is an
efficient method for the structural analysis of purified biological molecules. However, cryo …

Cryo-electron microscopy is an essential tool for high-resolution structural studies of
biological systems. This method relies on the use of phase contrast imaging at high defocus …

Advances in cryogenic transmission electron microscopy have revolutionised the
determination of many macromolecular structures at atomic or near-atomic resolution. This …

Electron ptychography has seen a recent surge of interest for phase sensitive imaging at
atomic or near-atomic resolution. However, applications are so far mainly limited to radiation …

Cryo electron tomography is a three‐dimensional imaging technique that is suitable for
imaging snapshots of the structural arrangements of biomolecular complexes and …

Cryo-electron microscopy techniques and computational 3-D reconstruction of
macromolecular assemblies are tightly linked tools in modern structural biology. This …

There has been enormous progress during the last few years in the determination of three-
dimensional biological structures by single particle electron cryomicroscopy (cryoEM) …

Highlights•Phase plates are an emerging technology in cryo-electron microscopy (cryo-
EM).•In cryo-electron tomography (cryo-ET) phase plates allow the visualization of cellular …

Electron tomography (ET) is a three-dimensional technique suitable to study pleomorphic
biological structures with nanometer resolution. This makes the methodology remarkably …