Non‐specific amplification compromises environmental DNA metabarcoding with COI

RA Collins, J Bakker, OS Wangensteen… - Methods in Ecology …, 2019 - Wiley Online Library
Metabarcoding extra‐organismal DNA from environmental samples is now a key technique
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …

Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems

A Polanco F, E Richards, B Flück, A Valentini… - Environmental …, 2021 - Wiley Online Library
Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring
of organisms is becoming easier and promises a revolution in our approaches to detect …

Why the COI barcode should be the community DNA metabarcode for the metazoa

C Andújar, P Arribas, DW Yu, AP Vogler, BC Emerson - 2018 - Wiley Online Library
Metabarcoding of complex metazoan communities is increasingly being used to measure
biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to …

[HTML][HTML] Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes

B Günther, T Knebelsberger, H Neumann… - Scientific Reports, 2018 - nature.com
We establish the new approach of environmental DNA (eDNA) analyses for the North Sea.
Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase …

[HTML][HTML] MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

[HTML][HTML] Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans

J Lobo, PM Costa, MAL Teixeira, MSG Ferreira… - BMC ecology, 2013 - Springer
Background Building reference libraries of DNA barcodes is relatively straightforward when
specifically designed primers are available to amplify the COI-5P region from a relatively …

Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization

GJ Jeunen, M Knapp, HG Spencer… - Ecology and …, 2019 - Wiley Online Library
DNA extraction from environmental samples (environmental DNA; eDNA) for metabarcoding‐
based biodiversity studies is gaining popularity as a noninvasive, time‐efficient, and cost …

[HTML][HTML] Validation and development of COI metabarcoding primers for freshwater macroinvertebrate bioassessment

V Elbrecht, F Leese - Frontiers in Environmental Science, 2017 - frontiersin.org
A central challenge in the present era of biodiversity loss is to assess and manage human
impacts on freshwater ecosystems. Macroinvertebrates are an important group for such …

Blind assessment of vertebrate taxonomic diversity across spatial scales by clustering environmental DNA metabarcoding sequences

V Marques, PÉ Guérin, M Rocle, A Valentini… - …, 2020 - Wiley Online Library
Human activities impact all ecosystems on Earth, which urges scientists to better understand
biodiversity changes across temporal and spatial scales. Environmental DNA (eDNA) …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …