Non‐specific amplification compromises environmental DNA metabarcoding with COI
Metabarcoding extra‐organismal DNA from environmental samples is now a key technique
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …
Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems
A Polanco F, E Richards, B Flück, A Valentini… - Environmental …, 2021 - Wiley Online Library
Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring
of organisms is becoming easier and promises a revolution in our approaches to detect …
of organisms is becoming easier and promises a revolution in our approaches to detect …
Why the COI barcode should be the community DNA metabarcode for the metazoa
Metabarcoding of complex metazoan communities is increasingly being used to measure
biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to …
biodiversity in terrestrial, freshwater and marine ecosystems, revolutionizing our ability to …
[HTML][HTML] Metabarcoding of marine environmental DNA based on mitochondrial and nuclear genes
We establish the new approach of environmental DNA (eDNA) analyses for the North Sea.
Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase …
Our study uses a multigene approach, including the mitochondrial cytochrome-c-oxidase …
[HTML][HTML] MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
[HTML][HTML] Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans
Background Building reference libraries of DNA barcodes is relatively straightforward when
specifically designed primers are available to amplify the COI-5P region from a relatively …
specifically designed primers are available to amplify the COI-5P region from a relatively …
Species‐level biodiversity assessment using marine environmental DNA metabarcoding requires protocol optimization and standardization
DNA extraction from environmental samples (environmental DNA; eDNA) for metabarcoding‐
based biodiversity studies is gaining popularity as a noninvasive, time‐efficient, and cost …
based biodiversity studies is gaining popularity as a noninvasive, time‐efficient, and cost …
[HTML][HTML] Validation and development of COI metabarcoding primers for freshwater macroinvertebrate bioassessment
V Elbrecht, F Leese - Frontiers in Environmental Science, 2017 - frontiersin.org
A central challenge in the present era of biodiversity loss is to assess and manage human
impacts on freshwater ecosystems. Macroinvertebrates are an important group for such …
impacts on freshwater ecosystems. Macroinvertebrates are an important group for such …
Blind assessment of vertebrate taxonomic diversity across spatial scales by clustering environmental DNA metabarcoding sequences
V Marques, PÉ Guérin, M Rocle, A Valentini… - …, 2020 - Wiley Online Library
Human activities impact all ecosystems on Earth, which urges scientists to better understand
biodiversity changes across temporal and spatial scales. Environmental DNA (eDNA) …
biodiversity changes across temporal and spatial scales. Environmental DNA (eDNA) …
Biases in bulk: DNA metabarcoding of marine communities and the methodology involved
LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …