When taking up the gauntlet of studying membrane protein functionality, scientists are
provided with a plethora of advantages, which can be exploited for the synthesis of these …

The preparation of sufficient amounts of high-quality samples is still the major bottleneck for
the characterization of membrane proteins by in vitro approaches. The hydrophobic nature …

Cell-free expression offers an interesting alternative method to produce membrane proteins
in high amounts. Elimination of toxicity problems, reduced proteolytic degradation and a …

Despite advances in membrane protein (MP) structural biology and a growing interest in
their applications, these proteins remain challenging to study. Progress has been hindered …

Cell-free expression has emerged as a powerful technique to overcome major restrictions of
classical in vivo membrane protein production, with sample yields of mgms of protein per ml …

Routine strategies for the cell-free production of membrane proteins in the presence of
detergent micelles and for their efficient co-translational solubilization have been developed …

The production of membrane proteins in cellular systems is besieged by several problems
due to their hydrophobic nature which often causes misfolding, protein aggregation and …

Cell-free expression has emerged as a promising tool for the fast and efficient production of
membrane proteins. The rapidly growing number of successfully produced targets in …

Cell-free protein production has become a core technology in the rapidly spreading field of
synthetic biology. In particular the synthesis of membrane proteins, highly problematic …

Cell-free expression techniques have emerged as promising tools for the production of
membrane proteins for structural and functional analysis. Elimination of toxic effects and a …