Genetically modified pigs for biomedical applications have been mainly generated using the
somatic cell nuclear transfer technique; however, this approach requires complex …

The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical
research. With the availability of genome editing tools such as clustered regularly …

The domestic pig has been widely used as an important large animal model. Precise and
efficient genetic modification in pig provides a great promise in biomedical research …

Genetically modified animals, especially rodents, are widely used in biomedical research.
However, non-rodent models are required for efficient translational medicine and preclinical …

Gene-edited pigs for agricultural and biomedical applications are typically generated using
somatic cell nuclear transfer (SCNT). However, SCNT requires the use of monoclonal cells …

Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient
gene-editing strategy for genome modification of different species. Genetic engineering in …

Xenoantigens cause hyperacute rejection and limit the success of interspecific xenografts.
Therefore, genes involved in xenoantigen biosynthesis, such as GGTA1, CMAH, and …

Pigs are an important resource in agriculture and serve as a model for human diseases. Due
to their physiological and anatomical similarities with humans, pigs can recapitulate …

The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by
several factors, including the mode of delivery, when applied to mammalian embryos. Given …

Genetically modified pigs are increasingly used for biomedical and agricultural applications.
The efficient CRISPR/Cas9 gene editing system holds great promise for the generation of …