G protein coupled receptors (GPCRs) are key players in signal recognition and cellular
communication making them important therapeutic targets. Large-scale production of these …

To facilitate purification and structural characterization, the CB2 cannabinoid receptor is
expressed in methylotrophic yeast Pichia pastoris. The expression plasmids were …

The overexpression of G protein‐coupled receptors (GPCRs) and of many other
heterologous membrane proteins in simple microbial hosts, such as the bacterium …

For the purpose of purification and structural characterization, the CB1 cannabinoid
receptors are expressed in methylotrophic yeast Pichia pastoris. The expression plasmid …

This study was conducted to optimize the expression of human CB2 cannabinoid receptors
in methylotrophic yeast Pichia pastoris (P. pastoris). Two major species of expressed CB2 …

Expression of milligram quantities of functional, stable G protein-coupled receptors (GPCR)
for high-resolution structural studies remains a challenging task. The goal of this work was to …

G protein‐coupled receptors (GPCRs) are notoriously difficult to express, particularly in
microbial systems. Using GPCR fusions with the green fluorescent protein (GFP), we …

Obtaining sufficient amount of purified G-protein coupled receptors (GPCRs) is almost
always one of the major challenges for their structural studies. CB2271–326, a human …

The human central CB1 and peripheral CB2 cannabinoid receptors were expressed in
Escherichia coli as fusion proteins with the maltose-binding protein at their amino-termini …

Human cannabinoid receptor CB 2 belongs to the class A of G protein-coupled receptor
(GPCR). CB 2 is predominantly expressed in membranes of cells of immune origin and is …