The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long
been used in cancer research. However, this system cannot be fully translated into clinical …

Abstract Three-dimensional (3D) culture systems are becoming increasingly popular due to
their ability to mimic tissue-like structures more effectively than the monolayer cultures. In …

Cancer cell lines have contributed immensely in understanding the complex physiology of
cancers. They are excellent material for studies as they offer homogenous samples without …

Cell culture is an important tool for biological research. Two-dimensional cell culture has
been used for some time now, but growing cells in flat layers on plastic surfaces does not …

Abstract Three-dimensional (3D) cell cultures have emerged as valuable tools in cancer
research, offering significant advantages over traditional two-dimensional (2D) cell culture …

Cell culture is a widely used in vitro tool for improving our understanding of cell biology,
tissue morphology, and mechanisms of diseases, drug action, protein production and the …

Three-dimensional (3D) tissue culture models have an invaluable role in tumour biology
today providing some very important insights into cancer biology. As well as increasing our …

Although historically, the traditional bidimensional in vitro cell system has been widely used
in research, providing much fundamental information regarding cellular functions and …

The re-creation of the tumor microenvironment including tumor–stromal interactions, cell–
cell adhesion and cellular signaling is essential in cancer-related studies. Traditional two …

A 3D cell culture has developed rapidly in recent years, as cells growing on a flat substrate
in a static environment are far from achieving an in vivo status. Currently, researchers have …